HtrA Stress Protein Is Involved in Intramacrophagic Replication of Adherent and Invasive Escherichia coli Strain LF82 Isolated from a Patient with Crohn's Disease
| Autor: | Arlette Darfeuille-Michaud, Anne-Lise Glasser, Marie-Agnès Bringer, Nicolas Barnich, Olivier Bardot |
|---|---|
| Přispěvatelé: | Institut National de la Santé et de la Recherche Médicale (INSERM), Microbes, Intestin, Inflammation et Susceptibilité de l'Hôte (M2iSH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA), Université d'Auvergne - Clermont-Ferrand I (UdA), Génétique, Reproduction et Développement - Clermont Auvergne (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), BARDOT, Olivier, Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Rok vydání: | 2005 |
| Předmět: |
Phagocytosis
Immunology lac operon medicine.disease_cause Microbiology 03 medical and health sciences Crohn Disease Escherichia coli PROTEINE PERIPLASMIQUE medicine Humans Promoter Regions Genetic Gene Heat-Shock Proteins 030304 developmental biology Phagosome Regulation of gene expression MALADIE DE CROHN 0303 health sciences SERINE ENDOPEPTIDASE biology 030306 microbiology Macrophages Serine Endopeptidases Promoter Gene Expression Regulation Bacterial biology.organism_classification Molecular Pathogenesis [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Enterobacteriaceae 3. Good health Oxidative Stress Infectious Diseases Mutation DNA Transposable Elements bacteria Parasitology PROTEINE DU STRESS Periplasmic Proteins [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Cell Division |
| Zdroj: | Infection and Immunity Infection and Immunity, American Society for Microbiology, 2005, 73 (2), pp.712-721. ⟨10.1128/IAI.73.2.712-721.2005⟩ Infection and Immunity, 2005, 73 (2), pp.712-721. ⟨10.1128/IAI.73.2.712-721.2005⟩ |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.73.2.712-721.2005 |
| Popis: | Adherent and invasive Escherichia coli (AIEC) bacteria isolated from Crohn's disease patients are able to greatly replicate within macrophages without escaping from the phagosome and without inducing macrophage death. In the present study, evidence is provided that in AIEC strain LF82 the htrA gene encoding the stress protein HtrA is essential for intracellular replication within J774-A1 macrophages. Deletion of the htrA gene in strain LF82 induced increased sensitivity of the isogenic mutant to oxidative stress caused by hydrogen peroxide and a reduced rate of growth in an acid and nutrient-poor medium partly reproducing the microenvironment of the phagosome. In vitro experiments using an LF82 htrA gene promoter fusion with the lacZ gene revealed a 38-fold activation of the promoter in AIEC LF82 intramacrophagic bacteria. The CpxRA two-component signaling pathway was not involved in this activation. In addition, the activation of the LF82 htrA gene promoter was not observed in the nonpathogenic E. coli K-12 intramacrophagic bacteria, indicating that the AIEC LF82 genetic background is crucial for induction of htrA gene transcription during phagocytosis. |
| Databáze: | OpenAIRE |
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