Selective involvement of the Fas (CD95) / Fas ligand pathway in bone marrow B cell progenitors
| Autor: | Géraldine Moreau, Corinne Garcia, Sophie Ezine, Yasmina Laouar, Florence Vasseur, Anne Claude Waché, Valérie Pasqualetto |
|---|---|
| Přispěvatelé: | Différenciation thymique et physiologie des lymphocytes T (Inserm U345), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), UMR 8603, Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5) |
| Rok vydání: | 2000 |
| Předmět: |
Fas Ligand Protein
Lymphocyte T cell Immunology Naive B cell Apoptosis Bone Marrow Cells Biology Fas ligand Mice medicine Animals Immunology and Allergy fas Receptor Progenitor cell B cell B-Lymphocytes Membrane Glycoproteins Cell Differentiation Fas receptor Molecular biology Mice Inbred C57BL medicine.anatomical_structure [SDV.IMM]Life Sciences [q-bio]/Immunology Signal Transduction |
| Zdroj: | European Journal of Immunology European Journal of Immunology, Wiley-VCH Verlag, 2000, 30 (5), pp.1402-1409. ⟨10.1002/(SICI)1521-4141(200005)30:53.0.CO;2-B⟩ |
| ISSN: | 1521-4141 0014-2980 |
| Popis: | International audience; B lymphocyte generation in bone marrow (BM) compensates for cell loses. The Fas / Fas ligand (FasL) pathway has been implicated in apoptosis of various cell types. Abnormalities of the Fas receptor or of FasL expression are associated with excessive T cell proliferation and autoimmunity. To examine the role of the Fas / FasL system in B cell differentiation, we created double‐chimeric mice by transferring both C57BL / 6 (B6)‐Fas+ and lpr‐FasL+ BM cells into RAG‐2– / – hosts. Equal numbers of stem cells were co‐injected into sublethally irradiated recipients, and their progeny were studied by using antibodies directed against the B6‐Ly5.1+5.2+ and lpr‐Ly5.1–5.2+ populations. A longitudinal study lasting for up to 6 months revealed that cells of the lpr phenotype dominated the B6 phenotype in the BM, as a result of their active proliferation. Analysis of the B cell compartment showed more lpr than B6 cells among immature HSAhiB220lo populations. In contrast, the lpr and B6 phenotypes were equally represented among mature B cells. BM transfer to second hosts indicated that B6‐derived B cell progenitors were absent from the first host. These data suggest that activation of the Fas / FasL pathway disturbs the early steps of B cell development and might therefore contribute to the onset of autoimmune disorders. |
| Databáze: | OpenAIRE |
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