Effectiveness of strategies to screen for blood donors with RH variants in a mixed population
| Autor: | Carla Luana Dinardo, Alexandre C. Pereira, Cecilia Salete Alencar, Fabio Luz, Fabio Augusto Abreu de Almeida, Vanderson Rocha, José Eduardo Krieger, Alfredo Mendrone-Junior, Ester Cerdeira Sabino, V.B. Oliveira, Marcia Regina Dezan |
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| Rok vydání: | 2019 |
| Předmět: |
Male
education.field_of_study Rh-Hr Blood-Group System biology African descent Population Blood Donors Hematology 030204 cardiovascular system & hematology Phenotype 03 medical and health sciences 0302 clinical medicine Antigen RH-antibodies Genotype Immunology biology.protein Humans Female Rh phenotype Antibody education 030215 immunology |
| Zdroj: | Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis. 59(2) |
| ISSN: | 1473-0502 |
| Popis: | Introduction Patients with RH variants presenting antibodies directed to RH high frequency antigens or multiple RH antibodies might, in some occasions, be better served with RH genotype-matched units, requiring screening for RH variants among blood donors. To date, strategies to identify donors with RH variants were restricted to selecting individuals of African descent based on self-reported race, what can be inaccurate in racially mixed population. Our goal was to: 1) Screen for donors with RH variants in a mixed population using self-declared race and Rh phenotype as selection criteria; and 2) Verify if including the Duffy null genotype in the screening algorithm increases its effectiveness. Methods Brazilian donors were included if self-declared as black and phenotyped as R0r or R1r. All individuals were genotyped for RHCE exons 1, 5, 6 and 7 and for the FY*B c.−67 T > C polymorphism in order to determine the Duffy null genotype. RHD variants were searched for in cases of altered RHCE. Results Among 2500 blood donors, 217 fulfilled the inclusion criteria and were enrolled. Fifty-three (24.4 %) had a predicted clinically relevant Rh phenotype (partial antigens or lack of high frequency antigens). Twelve donors (5.5 %) had a predicted RhCE phenotype lacking either hrB or hrS. Most cases with predicted lack of high frequency antigens (66.7 %) occurred in donors with the Duffy null genotype. Conclusion Selecting donors based on self-declared race, Rh phenotype and Duffy null genotype is feasible and effective in identifying RH variants lacking Rh high frequency antigens among racially mixed donors. |
| Databáze: | OpenAIRE |
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