Structural changes of cell wall and lignifying enzymes modulations in bean roots in response to copper stress
| Autor: | Anja Geitmann, Houda Bouazizi, Hager Jouili, Ezzeddine El Ferjani |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Copper Sulfate
Endocrinology Diabetes and Metabolism Clinical Biochemistry Phenylalanine ammonia-lyase Biochemistry Plant Roots Inorganic Chemistry Cell wall chemistry.chemical_compound Suberin Cell Wall Stress Physiological Lignin Phenylalanine Ammonia-Lyase Phaseolus Chemistry Biochemistry (medical) Callose food and beverages Xylem General Medicine Enzyme Activation Peroxidases Endodermis Phloem |
| Zdroj: | Biological trace element research. 136(2) |
| ISSN: | 1559-0720 |
| Popis: | Fourteen-day-old bean seedlings were cultured in nutrient solution containing Cu(2+) ions at various concentrations (50 and 75 microM of CuSO(4)) for 3 days. This excess of copper induced a reduction in the water volume absorbed by the plants. Moreover, this reduction was accompanied by an increase of the amount of copper taken up by the roots. Analysis by native gel electrophoresis of cell wall peroxidase activities in the roots revealed a stimulation of two anionic isoforms (A(2) and A(3)) under cupric stress conditions. Moreover, the activity of phenylalanine ammonia lyase (EC. 4.3.1.5), which plays an important role in plant defense, was enhanced. Copper-treated bean roots showed modifications in the cell walls of various tissues. Label for lignin, callose, and suberin with berberine-aniline blue revealed abnormal cell wall thickenings in the endodermis, the phloem, and the xylem, especially in plants treated with 75 microM CuSO(4). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink