Glucose Transport in Astrocytes: Regulation by Thyroid Hormone
| Autor: | J. T. Tildon, I B Williams, Roeder Lm |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
medicine.medical_specialty
Biological Transport Active Deoxyglucose Biology Biochemistry Cellular and Molecular Neuroscience Pregnancy Internal medicine Deoxy Sugars medicine Animals Incubation Cells Cultured Hexose transport Triiodothyronine Thyroid Glucose transporter Brain Rats Inbred Strains Metabolism Culture Media Rats Kinetics Endocrinology medicine.anatomical_structure Animals Newborn Bucladesine Astrocytes Female Astrocyte Hormone |
| Zdroj: | Journal of Neurochemistry. 45:1653-1657 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1111/j.1471-4159.1985.tb07239.x |
| Popis: | Primary cultures of astrocytes from newborn rat brain showed evidence of a substrate-saturable process for glucose transport. The system shows a relatively high affinity for the substrate, with an apparent Km of approximately 1 mM. Maintenance of the cells in medium containing thyroid-hormone-free serum for 3, 6, or 9 days resulted in significantly reduced rates of hexose transport. Addition of exogenous triiodothyronine to the transport incubation medium of these “hypothyroid′’ cells markedly increased the net rate of 2-deoxyglucose uptake within 60 s to values equal to or above those of control cultures (cells maintained in normal serum). These findings support a key role for thyroid hormone in the transport of glucose across plasma membranes of brain cells and demonstrate the presence of this regulatory system in astrocytes. |
| Databáze: | OpenAIRE |
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