Expression of matrix metalloproteinase-2 and metalloproteinase-9 in the skin of dogs with visceral leishmaniasis

Autor: Ana Paula Prudente Jacintho, Pamela Rodrigues Reina Moreira, Rosemeri de Oliveira Vasconcelos, Thiago A.C. de Souza, Guilherme D. Melo, Paulo Henrique Leal Bertolo, Claudia Momo, Gisele Fabrino Machado
Přispěvatelé: Rio Preto University Center (UNIRP), Pasteur Institute, Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP), Lyssavirus, épidémiologie et neuropathologie - Lyssavirus Epidemiology and Neuropathology, Institut Pasteur [Paris], University Centre of Rio Preto (UNIRP), Département Infection et Epidémiologie - Department of Infection and Epidemiology, Institut Pasteur [Paris] (IP), Universidade Estadual Paulista Júlio de Mesquita Filho = São Paulo State University (UNESP), Universidade de São Paulo = University of São Paulo (USP)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Pathology
Matrix metalloproteinase
Parasite load
Parasite Load
0403 veterinary science
Extracellular matrix
Abdomen
Dog
Dog Diseases
Lymphocytes
Leishmania infantum
ComputingMilieux_MISCELLANEOUS
Skin
Metalloproteinase
biology
Zymography
Ear
04 agricultural and veterinary sciences
General Medicine
Immunohistochemistry
3. Good health
Extracellular Matrix
Infectious Diseases
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Matrix Metalloproteinase 9
Leishmaniasis
Visceral
Matrix Metalloproteinase 2
medicine.medical_specialty
040301 veterinary sciences
Plasma Cells
Nose
Collagen Type I
03 medical and health sciences
Dogs
medicine
Animals
Inflammation
Mouth
General Veterinary
Macrophages
Gelatinases A and B
Leishmaniasis
medicine.disease
biology.organism_classification
030104 developmental biology
Visceral leishmaniasis
Collagen Type III
Insect Science
Parasitology
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Parasitology Research
Parasitology Research, Springer Verlag (Germany), 2018, 117 (6), pp.1819-1827. ⟨10.1007/s00436-018-5868-9⟩
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Parasitology Research, 2018, 117 (6), pp.1819-1827. ⟨10.1007/s00436-018-5868-9⟩
ISSN: 0932-0113
1432-1955
DOI: 10.1007/s00436-018-5868-9⟩
Popis: Made available in DSpace on 2018-12-11T17:19:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-06-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The skin is the first organ to be infected by the parasite in canine visceral leishmaniasis. The enzyme matrix metalloproteinase (MMP) acts towards degradation of the extracellular matrix (ECM) and modulation of the inflammatory response against many kinds of injuries. The aims of this study were to evaluate the expression of MMP-2 and MMP-9 through immunohistochemistry and zymography on the skin (muzzle, ears, and abdomen) of dogs that were naturally infected by Leishmania spp. and to compare these results with immunodetection of the parasite and with alterations to the dermal ECM. Picrosirius red staining was used to differentiate collagen types I and III in three regions of the skin. The parasite load, intensity of inflammation, and production of MMP-2 (latent) and MMP-9 (active and latent) were higher in the ear and muzzle regions. MMP-9 (active) predominated in the infected group of dogs and its production was significantly different to that of the control group. Macrophages, lymphocytes, and plasma cells predominated in the dermal inflammation and formed granulomas in association with degradation of mature collagen (type I) and with discrete deposition of young collagen (type III). This dermal change was more pronounced in dogs with high parasite load in the skin. Therefore, it was concluded that the greater parasite load and intensity of inflammation in the skin led consequently to increased degradation of mature collagen, caused by increased production of MMPs, particularly active MMP-9, in dogs with visceral leishmaniasis. This host response profile possibly favors systemic dissemination of the parasite. Department of Veterinary Medicine Rio Preto University Center (UNIRP) Department of Infection and Epidemiology (IE) Pasteur Institute Department of Clinical Medicine Surgery and Animal Reproduction Araçatuba School of Veterinary Medicine (FMVA) São Paulo State University (UNESP) Department of Veterinary Pathology School of Veterinary and Agrarian Sciences (FCAV) São Paulo State University (UNESP) Departamento de Patologia Veterinária FCAV - Universidade Estadual Paulista (UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n Department of Pathology School of Veterinary Medicine and Animal Science (FMVZ) University of São Paulo (USP) Department of Clinical Medicine Surgery and Animal Reproduction Araçatuba School of Veterinary Medicine (FMVA) São Paulo State University (UNESP) Department of Veterinary Pathology School of Veterinary and Agrarian Sciences (FCAV) São Paulo State University (UNESP) Departamento de Patologia Veterinária FCAV - Universidade Estadual Paulista (UNESP), Via de Acesso Prof. Paulo Donato Castellane, s/n FAPESP: 2009/07815-4
Databáze: OpenAIRE
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