Enterotoxin-Encoding Genes inStaphylococcusspp. from Food Handlers in a University Restaurant
Autor: | Thiago André Cidral, Maria Celeste Nunes de Melo, Sabina Dos Santos Paulino da Silva, Maria José dos Santos Soares |
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Rok vydání: | 2015 |
Předmět: |
Restaurants
Universities Food Handling Staphylococcus Microbial Sensitivity Tests Penicillins Bacitracin Enterotoxin Nose Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology law.invention Enterotoxins law medicine Humans Food science Polymerase chain reaction Food poisoning Hand medicine.disease Anti-Bacterial Agents Penicillin Gram staining Genes Bacterial Animal Science and Zoology Coagulase Brazil Food Science medicine.drug |
Zdroj: | Foodborne Pathogens and Disease. 12:921-925 |
ISSN: | 1556-7125 1535-3141 |
Popis: | Food handlers carrying enterotoxin-producing Staphylococcus are a potential source of food poisoning. The aim of this study was to analyze genes encoding enterotoxins in coagulase-positive Staphylococcus (CoPS) and coagulase-negative Staphylococcus (CoNS) isolated from the anterior nostrils and hands of food handlers at a university restaurant in the city of Natal, Northeast Brazil. Thirty food handlers were screened for the study. The isolates were subjected to Gram staining, a bacitracin sensitivity test, mannitol fermentation, and catalase and coagulase tests. CoNS and CoPS strains were subsequently identified by a Vitek 2 System (BioMerieux, France) and various biochemical tests. Polymerase chain reaction was used to detect genes for enterotoxins A, B, C, D, E, G, H, and I (sea, seb, sec, sed, see, seg, seh, and sei) and a disc-diffusion method was used to determine susceptibility to several classes of antimicrobials. All food handlers presented staphylococci on their hands and/or noses. The study found 58 Staphylococcus spp., of which 20.7% were CoPS and 79.3% were CoNS. S. epidermidis was the most prevalent species. Twenty-nine staphylococci (50%) were positive for one or more enterotoxin genes, and the most prevalent genes were seg and sei, each with a frequency of 29.3%. Indeed, CoNS encoded a high percentage of enterotoxin genes (43.5%). However, S. aureus encoded even more enterotoxin genes (75%). Most isolates showed sensitivity to the antibiotics used for testing, except for penicillin (only 35% sensitive). The results from this study reinforce that coagulase-negative as well as coagulase-positive staphylococci isolated from food handlers are capable of genotypic enterotoxigenicity. |
Databáze: | OpenAIRE |
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