Criteria for viability assessment of discarded human donor livers during ex vivo normothermic machine perfusion
| Autor: | Ton Lisman, Janneke Wiersema-Buist, Marieke T. de Boer, Negin Karimian, Annette S. H. Gouw, Michael E. Sutton, Robert J. Porte, Pepijn D. Weeder, Henri G. D. Leuvenink, Sanna op den Dries |
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| Přispěvatelé: | Groningen Institute for Organ Transplantation (GIOT), Vascular Ageing Programme (VAP) |
| Rok vydání: | 2014 |
| Předmět: |
Male
Pathology Time Factors medicine.medical_treatment BILE-SALT TOXICITY Liver transplantation Biochemistry chemistry.chemical_compound Oxidative Damage Medicine and Health Sciences Bile Multidisciplinary DEATH Hydrogen-Ion Concentration Middle Aged Tissue Donors Body Fluids medicine.anatomical_structure Liver Blood Chemistry Medicine GRAFTS Female Tissue Preservation Anatomy DUCTS Perfusion Artery Research Article medicine.medical_specialty Histology Bilirubin Science Urology Cold storage Surgical and Invasive Medical Procedures Biology Digestive System Procedures COLD-STORAGE BILIARY STRICTURES medicine INJURY Humans PRESERVATION Aged Demography Nutrition Machine perfusion Transplantation Biology and Life Sciences Nutrients Organ Transplantation Liver Transplantation Metabolism chemistry Biliary System Hepatocytes DONATION Bile Ducts Blood Gas Analysis Ex vivo Biomarkers |
| Zdroj: | PLoS ONE PLoS ONE, 9(11):e110642. PUBLIC LIBRARY SCIENCE PLoS ONE, Vol 9, Iss 11, p e110642 (2014) |
| ISSN: | 1932-6203 |
| Popis: | Although normothermic machine perfusion of donor livers may allow assessment of graft viability prior to transplantation, there are currently no data on what would be a good parameter of graft viability. To determine whether bile production is a suitable biomarker that can be used to discriminate viable from non-viable livers we have studied functional performance as well as biochemical and histological evidence of hepatobiliary injury during ex vivo normothermic machine perfusion of human donor livers. After a median duration of cold storage of 6.5 h, twelve extended criteria human donor livers that were declined for transplantation were ex vivo perfused for 6 h at 37 °C with an oxygenated solution based on red blood cells and plasma, using pressure controlled pulsatile perfusion of the hepatic artery and continuous portal perfusion. During perfusion, two patterns of bile flow were identified: (1) steadily increasing bile production, resulting in a cumulative output of ≥ 30 g after 6 h (high bile output group), and (2) a cumulative bile production |
| Databáze: | OpenAIRE |
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