Highly Sensitive, Specific Enzyme-Linked Immunosorbent Assay of Neopterin and Biopterin in Biological Samples
| Autor: | Ikuko Nagatsu, Takashi Sugimoto, K Fujita, Shoji Ogiwara, Kazutoshi Kiuchi, R. Teradaira, Toshiharu Nagatsu |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Quality Control
Antiserum biology Biochemistry (medical) Clinical Biochemistry Neopterin Biopterin Enzyme-Linked Immunosorbent Assay chemistry.chemical_compound Microtiter plate Biochemistry chemistry Reference Values immune system diseases Neoplasms biology.protein Humans Lupus Erythematosus Systemic Bovine serum albumin Hapten Quantitative analysis (chemistry) Peroxidase |
| Zdroj: | Clinical Chemistry. 38:1954-1958 |
| ISSN: | 1530-8561 0009-9147 |
| DOI: | 10.1093/clinchem/38.10.1954 |
| Popis: | An enzyme immunosorbent assay of neopterin and biopterin on a polystyrene microtiter plate has been developed. A conjugate of neopterin or biopterin to bovine serum albumin was used to raise a specific antiserum against neopterin or biopterin in rabbits. An incubation mixture of the antiserum and samples prepared from human serum underwent another antigen-antibody reaction with the hapten fixed on the microtiter plate. The amount of antibody bound to the fixed hapten, which is inverse to the amount of hapten in the sample, was determined by using anti-rabbit IgG-horseradish peroxidase conjugate in a usual manner by measuring absorbance at 490 nm after reaction with o-phenylenediamine and hydrogen peroxide. The minimal detectable amounts of neopterin and biopterin were approximately 0.1 pmol. The specificity of the assay was so high that the assay system for neopterin completely distinguished it from biopterin, as judged from the cross-reaction of 0.002%, and vice versa. The amounts of neopterin and biopterin in human serum determined by the present method agreed well with those determined by high-performance liquid chromatography. We used the present method to determine the concentrations of neopterin in serum from healthy control subjects and patients with cancers and systemic lupus erythematosus; the results were consistent with literature data. |
| Databáze: | OpenAIRE |
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