In vitro angiogenesis by human umbilical vein endothelial cells (HUVEC) induced by three-dimensional co-culture with glioblastoma cells
| Autor: | Andre Htay, Zhi-Jian Chen, Helen L. Fillmore, George T. Gillies, Wagner G. dos Santos, Milton M. Sholley, William C. Broaddus |
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| Rok vydání: | 2008 |
| Předmět: |
Vascular Endothelial Growth Factor A
Cancer Research Umbilical Veins Angiogenesis Blotting Western Umbilical vein Fibrin Neovascularization Glioma Cell Line Tumor medicine Humans biology Neovascularization Pathologic business.industry Brain Neoplasms Endothelial Cells medicine.disease In vitro Cell Hypoxia Coculture Techniques nervous system diseases Cell biology Blot Neurology Oncology Cell culture Immunology cardiovascular system biology.protein Neurology (clinical) medicine.symptom business Glioblastoma |
| Zdroj: | Journal of neuro-oncology. 92(2) |
| ISSN: | 1573-7373 |
| Popis: | Glioblastoma multiforme (GBM) is one of the most highly vascularized of all human tumors. Our objective was to characterize a 3-dimensional (3-D) in vitro angiogenesis model by co-culturing HUVEC and GBM cells, and to study the role of VEGF in mediating capillary tubule formation in this model. HUVEC-coated dextran beads were suspended in fibrin gel with human glioma cells on top. The number of sprouts and the length of the processes were measured. HUVEC can be induced to form sprouts and longer processes with lumens, in co-culture with glioma cells that secrete VEGF. Addition of exogenous VEGF enhances this effect. In the absence of glioma cells, many single HUVEC migrate away from the beads, without significant tubule formation. Hypoxia further stimulated sprout formation by 50–100%. Anti-VEGF neutralizing antibody suppressed HUVEC sprouting by 75% in co-culture with glioma cells. This 3-D in vitro co-culture system provides a robust and useful model for analysis of the major steps of glioma-induced angiogenesis. |
| Databáze: | OpenAIRE |
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