The RGD motif is involved in CD97/ADGRE5-promoted cell adhesion and viability of HT1080 cells
| Autor: | Wen-Ye Tjong, Hsi-Hsien Lin |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Integrins Angiogenesis Fibrosarcoma Amino Acid Motifs Cell Integrin lcsh:Medicine Apoptosis Article Receptors G-Protein-Coupled 03 medical and health sciences 0302 clinical medicine Antigens CD Cell Adhesion Tumor Cells Cultured medicine Humans lcsh:Science Cell adhesion Cell Aggregation Cell Proliferation RGD motif Multidisciplinary biology Chemistry Cell growth lcsh:R Intrinsic apoptosis Cadherins Cell aggregation Cell biology 030104 developmental biology medicine.anatomical_structure biology.protein lcsh:Q Oligopeptides 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-38045-w |
| Popis: | CD97/ADGRE5 is an adhesion G protein-coupled receptor (aGPCR) involved in tumor cell adhesion, migration, angiogenesis, and apoptosis. CD97 has been shown previously to stimulate angiogenesis by interacting with integrins on endothelial cells via an Arginine-Glycine-Aspartic acid (RGD) motif. In this report, the role of the RGD motif in tumor cell adhesion and apoptosis was investigated using a previously-established HT1080 cell-based system. We found that the RGD motif is critical in CD97-promoted cell adhesion, in part due to the up-regulation of αvβ5 and α2β1 integrins, and that CD97 mediates its anti-apoptotic effect in extrinsic apoptosis via RGD-dependent cell adhesion. In contrast, CD97-modulated anti-apoptotic effect in intrinsic apoptosis is mediated by RGD-independent, N-cadherin-induced homotypic cell aggregation. Hence, CD97 promotes tumorigenesis via RGD-dependent and -independent mechanisms. |
| Databáze: | OpenAIRE |
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