PACSIN, a brain protein that is upregulated upon differentiation into neuronal cells
| Autor: | Michael Leitges, Rita Lange, Gaby Vopper, Markus Plomann, Stephen W. Scheff, Mats Paulsson, Dagmar Barthels, Stanley A. Baldwin, Matthias Cramer, Harold Cremer, Uwe A.O. Heinlein |
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| Rok vydání: | 1998 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Protein Serine-Threonine Kinases Biology Biochemistry SH3 domain Substrate Specificity src Homology Domains Mice Ganglia Spinal Complementary DNA Animals Entorhinal Cortex Tissue Distribution Amino Acid Sequence Phosphorylation Casein Kinase II Gene Protein Kinase C Protein kinase C Adaptor Proteins Signal Transducing Gene Library Neurons Base Sequence Sequence Homology Amino Acid cDNA library Neuropeptides Age Factors Intracellular Signaling Peptides and Proteins Cell Differentiation Phosphoproteins Molecular biology Nerve Regeneration Up-Regulation Phosphoprotein Casein kinase 2 Cell Adhesion Molecules Signal Transduction Proto-oncogene tyrosine-protein kinase Src |
| Zdroj: | European Journal of Biochemistry. 256:201-211 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1046/j.1432-1327.1998.2560201.x |
| Popis: | To identify genes that are differentially expressed during self-repair processes in mouse brain, we screened a subtracted cDNA library enriched for brain-specific clones. One of these clones, H74, detected a 4.4-kb mRNA predominantly expressed in brain and dorsal root ganglia neurons. Expression increased continuously during the lifespan and the state of differentiation, but decreased after entorhinal-cortex lesion. A full-length cDNA clone was isolated from a cerebellum cDNA library and characterized. Se- quence analysis and database search revealed high sequence similarity to FAP52, a protein expressed in focal-adhesion contacts, and uncharacterized Echinococcus and Caenorhabditis elegans gene products. Furthermore, peptide sequences derived from human cDNA fragments showed up to 65 % sequence identity at the amino acid level. The presence of a C-terminal src homology 3 (SH3) domain and its phosphorylation by casein kinase 2 (CK2) and protein kinase C (PKC) imply a role in signaling. Here we demonstrate that the gene encodes a phosphoprotein, referred to as PACSIN, with a restricted spatial and temporal expression pattern. |
| Databáze: | OpenAIRE |
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