Studies on a peptide with red pigment-concentrating and hyperglycemic activity from the cephalic endocrine system of the honeybee, Apis mellifera
| Autor: | Lars Josefsson, William S. Herman, Michael D. Van Norstrand, Jens B. Carlsen |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Blood Glucose
Male medicine.medical_specialty animal structures Invertebrate Hormones Cockroaches Grasshoppers Endocrinology Internal medicine biology.animal Decapoda Hemolymph medicine Animals Adipokinetic hormone Cockroach biology Pigmentation fungi Proteolytic enzymes Biological activity Bees biology.organism_classification Shrimp Pyrrolidonecarboxylic Acid Biochemistry Animal Science and Zoology Biological Assay Head Oligopeptides Locust Periplaneta |
| Zdroj: | General and comparative endocrinology. 42(4) |
| ISSN: | 0016-6480 |
| Popis: | Shrimp red pigment-concentrations hormone (RPCH), locust adipokinetic hormone (AKH), and locust Compound I all elevate hemolymph lipid in locusts and concentrate pigment in shrimp erythrophores. In addition, RPCH and AKH both elevate hemolymph carbohydrate in cockroaches. Material with comparable biological activity has been reported in many insects, but not in representatives of the order Hymenoptera. Distilled water head extracts of the honeybe, Apis , exhibited red pigment-concentrating activity in the shrimp Leander and hyperglycemic activity in the cockroach Periplaneta . Chemical characterization of the active substance in Apis extracts showed that like RPCH, AKH, and Compound II, it was heat stable, soluble in butanol and methanol, degraded by proteolytic enzymes, and localized in the cephalic neuroendocrine system. Similarly, like RPCH and Compound II, but unlike AKH, the Apis substance eluted at 1.2 V t during Sephadex G-25 gel filtration. The data thus provide the initial demonstration that the endocrine system of at least one hymenopteran contains a peptide that is chemically and biologically comparable to the RPCH/AKH/Compound II group of hormonally active peptides. Furthermore, the results support the hypothesis that such peptides may be commonly used as hormones in holometabolic insects. In addition, pure Compound II was shonw for the first time to be hyperglycemic in the cockroach. |
| Databáze: | OpenAIRE |
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