Contrasting effect of dark-chilling on chloroplast structure and arrangement of chlorophyll-protein complexes in pea and tomato: plants with a different susceptibility to non-freezing temperature

Autor: Jan Henk Venema, Katarzyna Gieczewska, Borys Kierdaszuk, Agnieszka Mostowska, Wim J. Vredenberg, Małgorzata Rosiak, Izabela Rumak, Joanna Koziol-Lipinska, Maciej Garstka
Jazyk: angličtina
Rok vydání: 2007
Předmět:
Chlorophyll
cation-induced thylakoid stacking
Chloroplasts
pea
Plant Science
chilling-sensitive
tomato
chemistry.chemical_compound
Solanum lycopersicum
Laboratorium voor Plantenfysiologie
Chlorophyll fluorescence
Plant Proteins
confocal laser scanning microscopy
hill reaction activity
soybean genotypes
biology
EPS-3
food and beverages
Darkness
O-J-D-I-P chlorophyll fluorescence
Cold Temperature
Chloroplast
Biochemistry
Thylakoid
Electrophoresis
Polyacrylamide Gel
chlorophyll-protein complexes
Solanaceae
Laboratory of Plant Physiology
chilling tolerant
macromolecular substances
arabidopsis-thaliana
Photosystem I
photosynthetic electron-transport
dark-chilling stress
thylakoid membranes
parasitic diseases
Genetics
Cold acclimation
chloroplast and thylakoid membrane structure
cold-acclimation
fungi
Peas
biology.organism_classification
Electron transport chain
Spectrometry
Fluorescence
chemistry
photosystem-i
cucumber leaves
subsequent recovery
fatty-acid levels
Zdroj: Planta, 226(5), 1165-1181
Planta, 226(5), 1165-1181. Springer Verlag
Planta 226 (2007) 5
ISSN: 0032-0935
Popis: The effect of dark-chilling and subsequent photoactivation on chloroplast structure and arrangements of chlorophyll-protein complexes in thylakoid membranes was studied in chilling-tolerant (CT) pea and in chilling-sensitive (CS) tomato. Dark-chilling did not influence chlorophyll content and Chl a/b ratio in thylakoids of both species. A decline of Chl a fluorescence intensity and an increase of the ratio of fluorescence intensities of PSI and PSII at 120 K was observed after dark-chilling in thylakoids isolated from tomato, but not from pea leaves. Chilling of pea leaves induced an increase of the relative contribution of LHCII and PSII fluorescence. A substantial decrease of the LHCII/PSII fluorescence accompanied by an increase of that from LHCI/PSI was observed in thylakoids from chilled tomato leaves; both were attenuated by photoactivation. Chlorophyll fluorescence of bright grana discs in chloroplasts from dark-chilled leaves, detected by confocal laser scanning microscopy, was more condensed in pea but significantly dispersed in tomato, compared with control samples. The chloroplast images from transmission-electron microscopy revealed that dark-chilling induced an increase of the degree of grana stacking only in pea chloroplasts. Analyses of O-J-D-I-P fluorescence induction curves in leaves of CS tomato before and after recovery from chilling indicate changes in electron transport rates at acceptor- and donor side of PS II and an increase in antenna size. In CT pea leaves these effects were absent, except for a small but irreversible effect on PSII activity and antenna size. Thus, the differences in chloroplast structure between CS and CT plants, induced by dark-chilling are a consequence of different thylakoid supercomplexes rearrangements.
Databáze: OpenAIRE
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