Calcitonin secretion, C cell differentiation and proliferation during the spontaneous development of murine medullary thyroid carcinoma
| Autor: | S, Lausson, G E, Volle, M, Bourges, E, Pidoux, C, Borrel, G, Milhaud, M S, Moukhtar, A, Jullienne, F, Treilhou-Lahille |
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| Rok vydání: | 1995 |
| Předmět: |
Calcitonin
Male Pathology medicine.medical_specialty Mitotic index Parafollicular cell Population Immunocytochemistry In situ hybridization Biology Pathology and Forensic Medicine hemic and lymphatic diseases medicine Animals Thyroid Neoplasms education Molecular Biology education.field_of_study Thyroid Cell Differentiation Cell Biology General Medicine Calcitonin secretion Rats Disease Models Animal Microscopy Electron medicine.anatomical_structure Carcinoma Medullary Female Cell Division |
| Zdroj: | Virchows Archiv : an international journal of pathology. 426(6) |
| ISSN: | 0945-6317 |
| Popis: | Medullary thyroid carcinoma (MTC), a C cell neoplasm, synthesizes large amounts of calcitonin (CT), its biological marker. However, in some cases with a poor prognosis, MTC is associated with low basal CT levels owing to a decrease in the thyroid CT content. Using a murine model of human MTC, we studied the relationships between CT biosynthesis, C cell proliferation, and the circulating CT level during MTC progression. Cell proliferation was revealed by autoradiography of radioactive thymidine incorporation in dividing nuclei, after CT or CT mRNA detection by immunocytochemistry (ICC) or in situ hybridization (ISH). All rat thyroids showed a severe hyperplasia of C cells containing significant amounts of CT and CT mRNA, and a very low mitotic index. Tumours were found in 68% of the thyroids. In the strongly immunoreactive small nodules (ICC+), many labelled nuclei were observed. Subsequently some nodular cells, still containing detectable CT mRNA (ISH+), were not detected by immunocytochemistry (ICC-) owing to a dramatic decrease in secretory granules. Their mitotic index increased, and a rise of the basal CT plasma level was noted. These ISH+, ICC- tumour MTC cells represent a modified aggressive tumour C cell population exhibiting an increased ability to proliferate and were detected by the rise in the basal circulating CT level. |
| Databáze: | OpenAIRE |
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