Colorimetric determination of the activity of methyltransferase based on nicking enzyme amplification and the use of gold nanoparticles conjugated to graphene oxide
| Autor: | Zhimei Li, Jin Bu, Ting Pi, Xiao Zhang, Bao-Zhu Chi, Xiang-Juan Zheng, Rui-Hong Deng |
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| Rok vydání: | 2019 |
| Předmět: |
Detection limit
Graphene 010401 analytical chemistry Oxide Nanochemistry 02 engineering and technology Nicking enzyme Conjugated system 021001 nanoscience & nanotechnology 01 natural sciences Combinatorial chemistry 0104 chemical sciences Analytical Chemistry Nanomaterials law.invention chemistry.chemical_compound chemistry law Colloidal gold 0210 nano-technology |
| Zdroj: | Microchimica Acta. 186 |
| ISSN: | 1436-5073 0026-3672 |
| DOI: | 10.1007/s00604-019-3690-4 |
| Popis: | A method is described for the colorimetric determination of the activity of CpG methyltransferase (M.SssI). It is based on (a) the crosslinking effect between dsDNA-modified gold nanoparticles (AuNPs) and graphene oxide (GO), and (b) an amplification reaction with the aid of a nicking enzyme. To avoid the aggregation of AuNPs (which would produce false signals), a hairpin DNA was connected to the AuNPs. Thus, the red color of the solution (measured at 530 nm) increases linearly with the activity of M.SssI from 0.2 to 60 U·mL−1, and the limit of detection is 67 U·mL−1. This is superior to some reported strategies. The method was successfully applied to analyze spiked serum samples. Conceivably, it represents a powerful tool for use in drug development and diagnosis. |
| Databáze: | OpenAIRE |
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