Gene transcription ontogeny of hypothalamic-pituitary-thyroid axis development in early-life stage fathead minnow and zebrafish
| Autor: | Joe Swintek, Eric C. Randolph, Evy Verbueken, Ellen D. G. Michiels, Dries Knapen, Chloé Bars, Gerald T. Ankley, Isabelle J. Gabriëls, Anthony L. Schroeder, Daniel L. Villeneuve, Lucia Vergauwen, Jenna E. Cavallin, Krysta R. Fitzpatrick, Travis W. Saari, Jelena Periz-Stanacev, Evelyn Stinckens, Serina L. Robinson, Steven Van Cruchten |
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| Rok vydání: | 2018 |
| Předmět: |
Fish Proteins
0301 basic medicine Hypothalamo-Hypophyseal System endocrine system Transcription Genetic Cyprinidae Thyroid Gland Danio Embryonic Development DIO2 Article 03 medical and health sciences Endocrinology Species Specificity Transcription (biology) Thyroid peroxidase Animals Zebrafish Gene Principal Component Analysis biology biology.organism_classification Cell biology 030104 developmental biology Thyroid hormone receptor alpha Hormone receptor Larva biology.protein Animal Science and Zoology Human medicine |
| Zdroj: | General and comparative endocrinology |
| ISSN: | 0016-6480 |
| DOI: | 10.1016/j.ygcen.2018.05.001 |
| Popis: | The hypothalamic-pituitary-thyroid (HPT) axis is known to play a crucial role in the development of teleost fish. However, knowledge of endogenous transcription profiles of thyroid-related genes in developing teleosts remains fragmented. We selected two model teleost species, the fathead minnow ( Pimephales promelas ) and the zebrafish ( Danio rerio ), to compare the gene transcription ontogeny of the HPT axis. Control organisms were sampled at several time points during embryonic and larval development until 33 days post-fertilization. Total RNA was extracted from pooled, whole fish, and thyroid-related mRNA expression was evaluated using quantitative polymerase chain reaction. Gene transcripts examined included: thyrotropin-releasing hormone receptor ( trhr ), thyroid-stimulating hormone receptor ( tshr ), sodium-iodide symporter ( nis ), thyroid peroxidase ( tpo ), thyroglobulin ( tg ), transthyretin ( ttr ), deiodinases 1, 2, 3a, and 3b ( dio1, dio2, dio3a and 3b ), and thyroid hormone receptors alpha and beta ( thrα and β ). A loess regression method was successful in identifying maxima and minima of transcriptional expression during early development of both species. Overall, we observed great similarities between the species, including maternal transfer, at least to some extent, of almost all transcripts (confirmed in unfertilized eggs), increasing expression of most transcripts during hatching and embryo-larval transition, and indications of a fully functional HPT axis in larvae. These data will aid in the development of hypotheses on the role of certain genes and pathways during development. Furthermore, this provides a background reference dataset for designing and interpreting targeted transcriptional expression studies both for fundamental research and for applications such as toxicology. |
| Databáze: | OpenAIRE |
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