Persistent Effects of a Synthetic Androstene Derivative on Activities of 3/-Hydroxysteroid Dehydrogenase and Glucose-6-phosphate Dehydrogenase in Rats
| Autor: | William C. Yakovac, Alfred M. Bongiovanni, Allen S. Goldman |
|---|---|
| Rok vydání: | 1965 |
| Předmět: |
endocrine system
medicine.medical_specialty Stromal cell Tetrazolium Salts Glucosephosphate Dehydrogenase In Vitro Techniques Biology chemistry.chemical_compound Endocrinology Intestinal mucosa Corpus Luteum In vivo Pseudomonas Internal medicine Adrenal Glands medicine Animals Glucose-6-phosphate dehydrogenase Hyperplasia Histocytochemistry Adrenal cortex Ovary Hydroxysteroid Dehydrogenases In vitro Enzymes Rats medicine.anatomical_structure Liver chemistry Female Steroids Corpus luteum |
| Zdroj: | Endocrinology. 77:1105-1118 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo-77-6-1105 |
| Popis: | The present study reports the effects of a synthetic steroidal inhibitor, 2α-cyano-4,4,17α-trimethyl-androst-5-en-17β- ol-3-one, on the histochemical activities of the 3α-, 3β- and 17β-enzymes and of G-6-PD in rat tissues in vivo and in vitro and on the histochemical and biochemical activities of the steroid dehydrogenases extracted from Pseudomonas testosteroni, in vitro. This inhibitor in vivo ma rkedly reduces the highly active 3β- enzyme in adrenal cortical, corpora luteal and ovarian follicular and stromal cells. These effects are evident as early as 3 hr after a single injection. Concomitantly, the activity of G-6- PD is markedly increased in these same cells and in hepatic cells. Hyperplasia of the adrenal cortex and corpora lutea, reduction in activity of the 3β-enzyme and an increase in activity of G-6-PD are apparent in the respective tissues at 24 hr after the last injection and persist for at least 4 weeks. The lower levels of activities of the 3β-enzyme and the 17β-enzyme in the intestinal mu... |
| Databáze: | OpenAIRE |
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