Comparison of oxidative stress effects on senescence patterning of human adult and perinatal tissue-derived stem cells in short and long-term cultures
| Autor: | Francesco Alviano, Federica Facchin, Silvia Canaider, Margherita Maioli, Carlo Ventura, Miriam Romano, Alessia Impellizzeri, Eva Bianconi |
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| Přispěvatelé: | Facchin, Federica, Bianconi, Eva, Romano, Miriam, Impellizzeri, Alessia, Alviano, Francesco, Maioli, Margherita, Canaider, Silvia, Ventura, Carlo |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Senescence Cell Adipose tissue Biology Real-Time Polymerase Chain Reaction Human mesenchymal stem cells 03 medical and health sciences human mesenchymal stem cell Cyclin-dependent kinase medicine Humans Wharton Jelly Cells Cultured Cellular Senescence Cell Proliferation Oxidative stress-induced premature senescence Cell growth Mesenchymal stem cell Senescence-associated β-galactosidase activity Cell Differentiation Mesenchymal Stem Cells General Medicine beta-Galactosidase Hydrogen peroxide Cell biology Transplantation Oxidative Stress 030104 developmental biology medicine.anatomical_structure biology.protein Cell senescence Stem cell Resazurin-based assay Research Paper |
| Zdroj: | International Journal of Medical Sciences |
| Popis: | Human Mesenchymal Stem Cells (hMSCs) undergo senescence in lifespan. In most clinical trials, hMSCs experience long-term expansion ex vivo to increase cell number prior to transplantation, which unfortunately leads to cell senescence, hampering post-transplant outcomes.Hydrogen peroxide (H2O2) in vitro represents a rapid, time and cost-effective tool, commonly used as oxidative stress tantalizing the stem cell ability to cope with a hostile environment, recapitulating the onset and progression of cellular senescence.Here, H2O2 at different concentrations (ranging from 50 to 400 mu M) and time exposures (1 or 2 hours - h), was used for the first time to compare the behavior of human Adipose tissue-derived Stem Cells (hASCs) and human Wharton's Jelly-derived MSCs (hWJ-MSCs), as representative of adult and perinatal tissue-derived stem cells, respectively. We showed timely different responses of hASCs and hWJ-MSCs at low and high subculture passages, concerning the cell proliferation, the cell senescence-associated beta-Galactosidase activity, the capability of these cells to undergo passages, the morphological changes and the gene expression of tumor protein p53 (TP53, alias p53) and cyclin dependent kinase inhibitor IA (CDKN IA, alias p21) post H2O2 treatments.The comparison between the hASC and hWJ-MSC response to oxidative stress induced by H2O2 is a useful tool to assess the biological mechanisms at the basis of hMSC senescence, but it could also provide two models amenable to test in vitro putative anti-senescence modulators and develop anti-senescence strategies. |
| Databáze: | OpenAIRE |
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