Making sticky cells: effect of galactose and ferrous iron on the attachment of Leptospirillum ferrooxidans to mineral surfaces
| Autor: | Karlo Guerrero, Aminael Sánchez-Rodríguez, Paulina Aguirre, Axel Schippers, Juan Carlos Gentina |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Minerals Mineral Bacteria 030106 microbiology Galactose General Medicine Adhesion Biology Microbiology Culture Media Leptospirillum ferrooxidans Ferrous 03 medical and health sciences chemistry.chemical_compound Biopolymers 030104 developmental biology Extracellular polymeric substance chemistry Ferrous Compounds Food science Energy source Molecular Biology Chemical composition |
| Zdroj: | Research in Microbiology. 169:569-575 |
| ISSN: | 0923-2508 |
| DOI: | 10.1016/j.resmic.2018.08.005 |
| Popis: | The purpose of this study was to compare the efficacy of galactose and high initial ferrous iron concentrations as inducers for extracellular polymeric substances (EPS) production in planktonic cells of Leptospirillum ferrooxidans and to study cell attachment to a mineral surface in comparison to cells not exposed to such substances. L. ferrooxidans was successfully adapted to grow in a modified 9K medium at different concentrations of galactose (0.15, 0.25, 0.35%) and also at different initial ferrous iron concentrations (18, 27, 36 g/L), which are higher than 9K medium (9 g/L). The experiments were done in shake flasks using ferrous iron as energy source. A comparison of growth kinetics showed a decreasing of maximum specific growth rate of L. ferrooxidans with increasing concentrations of galactose and initial ferrous iron. The EPS content increased and the EPS chemical composition (relative abundance of carbohydrates, proteins and ferric iron) changed with increasing concentrations of galactose and initial ferrous iron. Results revealed that the increase of the bacterial adhesion rather depended on the chemical composition, i.e. relative abundance of the constituents of the EPS, than on the total amount of EPS. The EPS induced by galactose seemed to be “stickier” than the one induced by ferrous iron. Based on the results of this study it is proposed that galactose might enhance biooxidation processes which needs to be tested in future studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect