Evaluating the Antibacterial Properties of Polyacetylene and Glucosinolate Compounds with Further Identification of Their Presence within Various Carrot (Daucus carota) and Broccoli (Brassica oleracea) Cultivars Using High-Performance Liquid Chromatography with a Diode Array Detector and Ultra Performance Liquid Chromatography–Tandem Mass Spectrometry Analyses
| Autor: | Owen Kenny, Dilip K. Rai, E Sheehy, D. Walsh, Mohammad B. Hossain, L Hinds, Paul Evans, Michael Gaffney |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Falcarinol Glucosinolates Brassica High-performance liquid chromatography 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Chromatography detector Botany Chromatography High Pressure Liquid Chromatography Bacteria biology Plant Extracts Polyynes 04 agricultural and veterinary sciences General Chemistry biology.organism_classification 040401 food science Anti-Bacterial Agents Daucus carota 030104 developmental biology chemistry Sinigrin Glucosinolate Brassica oleracea Food Additives General Agricultural and Biological Sciences |
| Zdroj: | Journal of Agricultural and Food Chemistry. 65:7186-7191 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.7b02029 |
| Popis: | Ongoing consumer concerns over using synthetic additives in foods has strongly influenced efforts worldwide to source suitable natural alternatives. In this study, the antibacterial efficacy of polyacetylene and glucosinolate compounds was evaluated against both Gram positive and Gram negative bacterial strains. Falcarinol [minimum inhibitory concentration (MIC) = 18.8-37.6 μg/mL] demonstrated the best overall antibacterial activity, while sinigrin (MIC = 46.9-62.5 μg/mL) was the most active glucosinolate compound. High-performance liquid chromatography with a diode array detector analysis showed falcarinol [85.13-244.85 μg/g of dry weight (DW)] to be the most abundant polyacetylene within six of the eight carrot (Daucus carota) cultivars investigated. Meanwhile, sinigrin (100.2-244.3 μg/g of DW) was the most abundant glucosinolate present within the majority of broccoli (Brassica oleracea) cultivars investigated using ultra performance liquid chromatography-tandem mass spectrometry analysis. The high abundance of both falcarinol and sinigrin within these respective species suggests that they could serve as potential sources of natural antibacterial agents for use as such in food products. |
| Databáze: | OpenAIRE |
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