Characterization of in Vitro Modified Human Very Low-Density Lipoprotein Particles and Phospholipids by Capillary Electrophoresis
| Autor: | Wei-Ting Lai, Yi-Jyun Lin, You-Sian Lin, Mine-Yine Liu, Mei-Yu Su, Yen-Yi Chen, Chin-Pong Chong, Yi-Han Liao, Yi-Ning Liu, Ting-Yu Shu, Huai-Guang Xie |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Glycation End Products
Advanced Very low-density lipoprotein Glycosylation Phospholipid In Vitro Techniques Lipoproteins VLDL digestive system Catalysis Micellar electrokinetic chromatography Article Inorganic Chemistry Absorbance lcsh:Chemistry chemistry.chemical_compound Capillary electrophoresis Glycation polycyclic compounds Humans micellar electrokinetic chromatography Physical and Theoretical Chemistry Molecular Biology lcsh:QH301-705.5 Spectroscopy phospholipids Chromatography Chemistry Organic Chemistry solid phase extraction Electrophoresis Capillary nutritional and metabolic diseases General Medicine in vitro oxidation Computer Science Applications Electrophoresis Glucose lcsh:Biology (General) lcsh:QD1-999 capillary zone electrophoresis in vitro glycation lipids (amino acids peptides and proteins) Oxidation-Reduction Lipoprotein very low-density lipoprotein |
| Zdroj: | International Journal of Molecular Sciences Volume 13 Issue 12 Pages 16400-16417 International Journal of Molecular Sciences, Vol 13, Iss 12, Pp 16400-16417 (2012) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms131216400 |
| Popis: | A simple capillary zone electrophoresis (CZE) method was used to characterize human very low-density lipoprotein (VLDL) particles for four healthy donors. One major peak was observed for native, in vitro oxidized and glycated VLDL particles. The effective mobilities and peak areas of the capillary electrophoresis (CE) profiles showed good reproducibility and precision. The mobility of the oxidized VLDL peak was higher than that of the native VLDL. The mobility of the glycated VLDL peak was similar to that of the native VLDL. Phospholipids isolated from VLDL particles were analyzed by our recently developed micellar electrokinetic chromatography (MEKC) with a high-salt stacking method. At absorbance 200 nm, the native VLDL phospholipids showed a major peak and a minor peak for each donor. For oxidized VLDL phospholipids, the area of the major peak reduced for three donors, possibly due to phospholipid decomposition. For glycated VLDL phospholipids, the peak mobilities were more positive than native VLDL phospholipids for two donors, possibly due to phospholipid-linked advanced glycation end products (AGEs). Very interestingly, at absorbance 234 nm, the major peak of oxidized VLDL phospholipids was resolved as two peaks for each donor, possibly due to conjugated dienes formed upon oxidation. |
| Databáze: | OpenAIRE |
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