Marked Stepwise Differences within a Common Kinetic Mechanism Characterize TATA-binding Protein Interactions with Two Consensus Promoters
| Autor: | Michael Brenowitz, Kay M. Parkhurst, Robyn M. Powell, Lawrence J. Parkhurst |
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| Rok vydání: | 2001 |
| Předmět: |
Time Factors
genetic processes RNA polymerase II Saccharomyces cerevisiae macromolecular substances Mole fraction Biochemistry Dissociation (chemistry) chemistry.chemical_compound Promoter Regions Genetic Molecular Biology Conformational isomerism biology Temperature Promoter DNA Cell Biology TATA-Box Binding Protein DNA-Binding Proteins Kinetics Spectrometry Fluorescence Förster resonance energy transfer Models Chemical chemistry biology.protein Biophysics Thermodynamics TATA-binding protein Adenovirus E4 Proteins Protein Binding Transcription Factors |
| Zdroj: | Journal of Biological Chemistry. 276:29782-29791 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m104099200 |
| Popis: | Binding of the TATA-binding protein (TBP) to promoter DNA bearing the TATA sequence is an obligatory initial step in RNA polymerase II transcription initiation. The interactions of Saccharomyces cerevisiae TBP with the E4 (TATATATA) and adenovirus major late (TATAAAAG) promoters have been modeled via global analysis of kinetic and thermodynamic data obtained using fluorescence resonance energy transfer. A linear two-intermediate kinetic mechanism describes the reaction of both of these consensus strong promoters with TBP. Qualitative features common to both interactions include tightly bound TBP-DNA complexes with similar solution geometries, simultaneous DNA binding and bending, and the presence of intermediate TBP-DNA conformers at high mole fraction throughout most of the reaction and at equilibrium. Despite very similar energetic changes overall, the stepwise entropic and enthalpic compensations along the two pathways differ markedly following the initial binding/bending event. Furthermore, TBP-E4 dissociation ensues from both replacement and displacement processes, in contrast to replacement alone for TBP-adenovirus major late promoter. A model is proposed that explicitly correlates these similarities and differences with the sequence-specific structural properties inherent to each promoter. This detailed mechanistic comparison of two strong promoters interacting with TBP provides a foundation for subsequent comparison between consensus and variant promoter sequences reacting with TBP. |
| Databáze: | OpenAIRE |
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