In vitro metabolomic footprint of the Echinococcus multilocularis metacestode
| Autor: | Britta Lundström-Stadelmann, Dominic Ritler, Urs Kämpfer, Claudia Bühr, Reto Rufener, Jia V. Li, Stefan Schürch, Joachim Müller |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Proton Magnetic Resonance Spectroscopy lcsh:Medicine Echinococcus multilocularis Biochemistry Microbiology Article Host-Parasite Interactions 03 medical and health sciences Metabolomics Drug Development Echinococcosis Zoonoses 540 Chemistry Parasite hosting Animals Humans Threonine lcsh:Science Author Correction Alanine chemistry.chemical_classification Multidisciplinary biology 630 Agriculture lcsh:R Anticestodal Agents 030108 mycology & parasitology biology.organism_classification In vitro 3. Good health Amino acid Metacestode 030104 developmental biology chemistry Larva 570 Life sciences lcsh:Q Parasitology |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-13 (2019) Ritler, Dominic; Rufener, Reto; Li, Jia V.; Kämpfer, Urs; Müller, Joachim; Bühr, Claudia; Schürch, Stefan; Lundström Stadelmann, Britta (2019). In vitro metabolomic footprint of the Echinococcus multilocularis metacestode. Scientific reports, 9(1), p. 19438. Springer Nature 10.1038/s41598-019-56073-y |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-56073-y |
| Popis: | Alveolar echinococcosis (AE) is a zoonotic disease that is deadly if left untreated. AE is caused by the larval metacestode stage of the cestode Echinococcus multilocularis. Better knowledge on the host-parasite interface could yield novel targets for improvement of the treatment against AE. We analyzed culture media incubated with in vitro grown E. multilocularis metacestodes by 1H nuclear magnetic resonance spectroscopy to identify the unknown metabolic footprint of the parasite. Moreover, we quantitatively analyzed all amino acids, acetate, glucose, lactate, and succinate in time-course experiments using liquid chromatography and enzymatic assays. The E. multilocularis metacestodes consumed glucose and, surprisingly, threonine and produced succinate, acetate, and alanine as major fermentation products. The metabolic composition of vesicle fluid (VF) from in vitro grown E. multilocularis metacestodes was different from parasite-incubated culture medium with respect to the abundance, but not the spectrum, of metabolites, and some metabolites, in particular amino acids, accumulated in the VF. Overall, this study presents the first characterization of the in vitro metabolic footprint of E. multilocularis metacestodes and VF composition, and it provides the basis for analyses of potentially targetable pathways for future drug development. |
| Databáze: | OpenAIRE |
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