Characterization of a mutant pancreatic eIF-2alpha kinase, PEK, and co-localization with somatostatin in islet delta cells
| Autor: | Jie An, Stramm Lawrence E, Jingdong Liang, George E. Sandusky, Na N. Yang, Scott E. Hayes, Yuguang Shi |
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| Rok vydání: | 1999 |
| Předmět: |
DNA
Complementary Molecular Sequence Data macromolecular substances Biology environment and public health Biochemistry Islets of Langerhans eIF-2 Kinase medicine Protein biosynthesis Animals Humans Point Mutation Amino Acid Sequence Kinase activity Phosphorylation Caenorhabditis elegans Molecular Biology Delta cell Base Sequence Sequence Homology Amino Acid Kinase Pancreatic islets Autophosphorylation Cell Biology Protein kinase R Cell biology Rats enzymes and coenzymes (carbohydrates) medicine.anatomical_structure Mutagenesis Site-Directed Somatostatin |
| Zdroj: | The Journal of biological chemistry. 274(9) |
| ISSN: | 0021-9258 |
| Popis: | Phosphorylation of eukaryotic translation initiation factor-2alpha (eIF-2alpha) is one of the key steps where protein synthesis is regulated in response to changes in environmental conditions. The phosphorylation is carried out in part by three distinct eIF-2alpha kinases including mammalian double-stranded RNA-dependent eIF-2alpha kinase (PKR) and heme-regulated inhibitor kinase (HRI), and yeast GCN2. We report the identification and characterization of a related kinase, PEK, which shares common features with other eIF-2alpha kinases including phosphorylation of eIF-2alpha in vitro. We show that human PEK is regulated by different mechanisms than PKR or HRI. In contrast to PKR or HRI, which are dependent on autophosphorylation for their kinase activity, a point mutation that replaced the conserved Lys-614 with an alanine completely abolished the eIF-2alpha kinase activity, whereas the mutant PEK was still autophosphorylated when expressed in Sf-9 cells. Northern blot analysis indicates that PEK mRNA was predominantly expressed in pancreas, though low expression was also present in several tissues. Consistent with the high levels of mRNA in pancreas, the PEK protein was only detected in human pancreatic islets, and the kinase co-localized with somatostatin, a pancreatic delta cell-specific hormone. Thus PEK is believed to play an important role in regulating protein synthesis in the pancreatic islet, especially in islet delta cells. |
| Databáze: | OpenAIRE |
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