A lavender ABC transporter confers resistance to monoterpene toxicity in yeast
Autor: | Soheil S. Mahmoud, Mike Tarnowycz, Zerihun A. Demissie, Ayelign M. Adal, Lukman S. Sarker |
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Rok vydání: | 2018 |
Předmět: |
surface charge
0106 biological sciences 0301 basic medicine Lavandula Lavender oil ATP-binding cassette transporter Saccharomyces cerevisiae Plant Science immunogenicity 01 natural sciences law.invention 03 medical and health sciences chemistry.chemical_compound law Genetics Essential oil Nanomaterials reactive oxygen species Cellulose nanocrystals Transporter NLRP3 inflammasome Drug Resistance Multiple Yeast Multiple drug resistance 030104 developmental biology chemistry Biochemistry Monoterpenes ATP-Binding Cassette Transporters Geraniol 010606 plant biology & botany |
Zdroj: | Planta. 249:139-144 |
ISSN: | 1432-2048 0032-0935 |
Popis: | The interest in functionalized cellulose nanocrystals (CNCs) for multiple biomedical application has been increasing in recent years. CNCs are suitable to functionalization with an array of polymers, generating chemically related nanomaterials with different morphologies, surface charges that can affect bioreactivity, including immune response. In this study, we sought to understand the mechanistic differences regarding immunological responses evoked by functionalized CNCs and whether surface charges play a role in this effect. We investigated the effect of a cationic, CNCs-poly(APMA), and an anionic, CNCs-poly(NIPAAm) derivatives on the secretion of inflammatory cytokines, mitochondria-derived ROS and mitochondrial function and antioxidant response as well as on endoplasmic reticulum (ER) stress, in human and murine inflammatory cells. The cationic CNCs-poly(APMA) evoked a more robust immunological response in murine cell line, while the anionic CNCs-poly(NIPAAm) showed a significant NLRP3 inflammasome-dependent and independent immunological response in human monocytes. Moreover, CNCs-poly(NIPAAm) induced greater formation of acidic vesicular organelles, mitochondrial ROS in non-stimulated cells while CNCs-poly(APMA) mainly affected mitochondrial function by decreasing the intracellular ATP. The differences on the biological responses may be related to the surface charges of CNCs, and their likely interactions with intra and extracellular biomolecules. |
Databáze: | OpenAIRE |
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