Identification of a novel cis-regulatory element for UV-B-induced transcription in Arabidopsis

Autor: Agnieszka Stec, Balázs Fehér, Giorgio Morelli, Geza Dallmann, Attila Oravecz, Andrea Ciolfi, Judit Safrany, Zoltán Máté, Ferenc Nagy, Roman Ulm, Veronika Haasz
Jazyk: angličtina
Rok vydání: 2008
Předmět:
Transcription
Genetic
arabidopsis
arabidopsis proteins
base sequence
genetic
genetics
genetics/metabolism
light
molecular sequence data
point mutation
promoter regions
radiation effects
regulatory elements
transcription
transcriptional
ultraviolet rays
Light
Ultraviolet Rays
Ubiquitin-Protein Ligases
Mutant
Molecular Sequence Data
Arabidopsis
Plant Science
Chimeric gene
Arabidopsis/genetics/metabolism
Biology
Transcription (biology)
Gene expression
Genetics
Point Mutation
Regulatory Elements
Transcriptional
Promoter Regions
Genetic
Gene
Transcription factor
Arabidopsis Proteins/genetics/metabolism
Base Sequence
Arabidopsis Proteins
Promoter
Cell Biology
Promoter Regions
Genetic/genetics
Cell biology
Regulatory sequence
Regulatory Elements
Transcriptional/genetics
Transcription
Genetic/radiation effects
Zdroj: Plant Journal, Vol. 54, No 3 (2008) pp. 402-414
ISSN: 0960-7412
Popis: Ultraviolet-B light (UV-B) regulates the expression of genes in a wavelength- and fluence rate-dependent fashion. A signaling pathway consisting of CONSTITUTIVE PHOTOMORPHOGENESIS 1 (COP1) and UV RESISTANCE LOCUS 8 (UVR 8) mediates responsiveness to longer wavelength, low intensity UV-B light-activating, for example, HY5 gene expression. By contrast, transcription of another group of genes, including ANAC13, modulated by shorter wavelength, higher intensity UV-B is controlled by a yet unknown and largely COP1-independent signaling cascade. Here we provide evidence by promoter deletion analysis, and characterization of genetic mutants displaying aberrant expression patterns, that two cis-regulatory elements, designated MRE(ANAC13) and UVBox(ANAC13), are required for maximal UV-B induction of the ANAC13 gene in transgenic plants. These elements are located in the proximal 150-bp region of the ANAC13 promoter. They show no significant similarity to each other; the putative MRE(ANAC13) (-AACCTT-) is closely related to MRE(CHS) (-AACCTA-) found in the CHALCONE SYNTHASE (CHS) gene, whereas UVBox(ANAC13) (with core sequence CAAG) represents a novel cis-regulatory element. The novel UVBox(ANAC13) sequence is significantly enriched in the promoter region of a subset of UV-B-induced genes with similar activation properties as ANAC13. In addition, we demonstrate that expression of a chimeric gene containing only the dimerized 12-mer containing UVBox(ANAC13) fused to a minimal CaMV35S promoter/luciferase reporter is (i) efficiently induced by shorter wavelength, higher intensity UV-B, but (ii) does not respond either to longer wavelength UV-B and red light or (iii) to abscisic acid treatment and osmotic, salt, heat and cold stresses.
Databáze: OpenAIRE
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