Maillard Reaction Induced Lactose Attachment to Bovine β-Lactoglobulin: Electrospray Ionization and Matrix-Assisted Laser Desorption/Ionization Examination
| Autor: | French Sj, Nan M. Kleinholz, Harper Wj, Jones Rb, Kari B. Green-Church |
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| Rok vydání: | 2002 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Electrospray Time Factors Electrospray ionization Lactose Lactoglobulins Mass spectrometry chemistry.chemical_compound symbols.namesake Desorption Ionization Animals Chromatography Chemistry Temperature Humidity General Chemistry Maillard Reaction Maillard reaction Matrix-assisted laser desorption/ionization Solubility Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization symbols Cattle General Agricultural and Biological Sciences |
| Zdroj: | Journal of Agricultural and Food Chemistry. 50:820-823 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf010571q |
| Popis: | Nonenzymatic attachment of lactose to β-lactoglobulin (β-Lg) was investigated under different conditions. Solubilized conditions, dry environment, and a combination of dry and solubilized environments, were examined for their effects on lactosylation. Temperatures ranging from 50 to 65 °C and time intervals between 1 h and 4 days were used. Electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry were implemented to examine the reaction products. Maximum attachment efficiency occurred at 65 °C held for 3 h in dry-way conditions. Incubations held for long periods of time under dry-way conditions suggest possible denaturation. Both ESI and MALDI data suggest β-Lg removal in the solubilized samples held for long periods of time. A combination of solubilized and dry environments led to very similar mass spectrogram results over time. Keywords: β-Lactoglobulin; nonenzymatic; solubilized; dry-way; combination |
| Databáze: | OpenAIRE |
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