A conjoint multi metal-ion iminodiacetic acid monolith microfluidic chip for structural-based protein pre-fractionation

Autor: Kishore K.R. Tetala, Mookambeswaran A. Vijayalakshmi, Ashish Khaparde, S. Lokesh Kumar
Rok vydání: 2021
Předmět:
Zdroj: Electrophoresis5 References. 42(24)
ISSN: 1522-2683
Popis: PDMS based multi-channel microfluidic chip was designed and fabricated in a simple approach using readily available tools. UV-initiated in situ polymerization of poly(2-hydroxy ethyl methacrylate-co-di(ethylene glycol) diacrylate-co-N,N'-diallyl L-tartardiamide) in an eppendorf tube was achieved within 40 min. This polymerization process was successfully translated to a microfluidic chip format without any further modifications. Iminodiacetic acid was successfully immobilized on aldehyde functional monoliths via Schiff base reaction and confirmed by FT-IR spectroscopy. Four transition metal-ions (Co (II), Zn (II), Ni (II), and Cu (II)) were chelated individually on four IDA-monolith microfluidic chips. The conjoint metal-ion monolith microfluidic chip has displayed high permeability (9.40 × 10-13 m2 ) and a porosity of 32.8%. This affinity microfluidic chip has pre-fractioned four human plasma proteins (fibrinogen, immunoglobulin, transferrin and human serum albumin) based on their surface exposed histidine surface topography. A protein recovery of ∼95% (Bradford assay data) was achieved. The multi-monolith microchip can be reusable even after 3 protein adsorption-desorption cycles. This article is protected by copyright. All rights reserved.
Databáze: OpenAIRE
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