Oocyte polarized light microscopy, assay of specific follicular fluid metabolites, and gene expression in cumulus cells as different approaches to predict fertilization efficiency after ICSI

Autor: Valentina Biasoni, Nicola Colacurci, Stefano Canosa, Alberto Revelli, Andrea Carosso, Milena Maule, Chiara Benedetto, Angela Bertagna, Loredana Bergandi, Francesca Evangelista, Maria Diletta D’Eufemia, Oleksii A. Skorokhod, Elisabetta Aldieri
Přispěvatelé: Revelli, Alberto, Canosa, Stefano, Bergandi, Loredana, Skorokhod, Oleksii A., Biasoni, Valentina, Carosso, Andrea, Bertagna, Angela, Maule, Milena, Aldieri, Elisabetta, D'Eufemia, Maria Diletta, Evangelista, Francesca, Colacurci, Nicola, Benedetto, Chiara
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Anti-Mullerian Hormone
Oocyte
Sperm Injections
Oocyte Retrieval
Gene Expression
0302 clinical medicine
Human fertilization
Endocrinology
Transforming Growth Factor beta
Polarization
Gene expression
Receptors
Amh
lcsh:Reproduction
Microscopy
030219 obstetrics & reproductive medicine
biology
Chemistry
Obstetrics and Gynecology
Anti-Müllerian hormone
Embryo
Intracytoplasmic
Cumulus cell
medicine.anatomical_structure
Peptide
Polarized light microscopy
Female
Microscopy
Polarization
Adult
medicine.medical_specialty
endocrine system
Receptors
Peptide
lcsh:QH471-489
Cumulus cells
AMH receptor type II
Embryonic Development
Fertilization
Follicular fluid
Oxidative stress
Cumulus Cells
Follicular Fluid
Humans
Oocytes
Receptors
Transforming Growth Factor beta
Sperm Injections
Intracytoplasmic
Reproductive Medicine
Developmental Biology
lcsh:Gynecology and obstetrics
Andrology
03 medical and health sciences
Follicle
Internal medicine
medicine
lcsh:RG1-991
urogenital system
Research
Embryogenesis
030104 developmental biology
biology.protein
Oxidative stre
Zdroj: Reproductive Biology and Endocrinology, Vol 15, Iss 1, Pp 1-12 (2017)
Reproductive Biology and Endocrinology : RB&E
ISSN: 1477-7827
Popis: Background The complex relationship between oocyte morphology, specific follicular fluid metabolites, gene expression in cumulus granulosa cells, and oocyte competence toward fertilization and embryo development still needs further clarification. Methods Forty-six oocytes retrieved from the largest pre-ovulatory follicle of patients undergoing intra-cytoplasmic sperm injection (ICSI) were considered assessing: (a) oocyte morphological characteristics at polarized light microscopy (PLM), (b) specific follicular fluid (FF) metabolites previously suggested to influence oocyte competence (AMH, markers of redox status and of cytotoxicity), (c) transcription of AMH and AMH type II receptor genes in cumulus cells. Data were analyzed using mono-parametric tests and multivariable logistic analysis in order to correlate morphological and biochemical data with fertilization. Results Comparing normally fertilized oocytes (n = 29, F group) with unfertilized (n = 17, nF group) we observed that: (a) the meiotic spindle area and major axis were significantly higher in nF group and in fertilized oocytes undergoing an early embryo development arrest; (b) AMH level in FF was comparable in F and nF groups; (c) the FF of nF group contained significantly higher levels of cytotoxicity (lactate dehydrogenase) and oxidative stress (Cu,Zn-superoxide dismutase, catalase, 4-hydroxynonenal-protein conjugates) markers; (d) cumulus cells of nF group showed significantly higher AMH receptor type II gene expression. Conclusions Taken together, these observations suggest that an excessive cytotoxicity level can alter AMH signal transduction within cumulus cells, in turn leading to partial inhibition of aromatase activity, altered cytoplasmic maturation and increased oxidative stress, factors able to impair oocyte fertilization competence and embryo growth. Electronic supplementary material The online version of this article (doi:10.1186/s12958-017-0265-2) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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