Determination of ractopamine and salbutamol in pig hair by liquid chromatography tandem mass spectrometry
| Autor: | Chin-En Tsai, Yu-Ting Chang, Kai-Chun Chang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Calibration curve Swine Electrospray ionization lcsh:TX341-641 Mass spectrometry 01 natural sciences chemistry.chemical_compound Liquid chromatography–mass spectrometry Limit of Detection Tandem Mass Spectrometry 010608 biotechnology Phenethylamines Animals Albuterol Solid phase extraction Chromatography High Pressure Liquid Pharmacology Detection limit Chromatography Chemistry 010401 analytical chemistry Hair analysis lcsh:RM1-950 Solid Phase Extraction Adrenergic beta-Agonists 0104 chemical sciences Ractopamine lcsh:Therapeutics. Pharmacology lcsh:Nutrition. Foods and food supply Food Science Hair |
| Zdroj: | Journal of Food and Drug Analysis, Vol 26, Iss 2, Pp 725-730 (2018) |
| ISSN: | 2224-6614 |
| Popis: | A liquid chromatography tandem mass spectrometric method was developed for the determination of two β-agonists (ractopamine and salbutamol) in pig hair samples. An isotope of ractopamine-d5 or salbutamol-d6 as an internal standard was used to carry out quantitative analysis. Concentrated sodium hydroxide was used to pretreat hair samples and then purified by the solid phase extraction (SPE) procedure. The extracted solution was evaporated and reconstituted for injection in the instrument with electrospray ionization (ESI) operating in a positive multiple-reaction-monitoring (MRM) mode. Ractopamine and salbutamol separation were performed on C18 analytical column under gradient condition. The internal standard calibration curve was linear in the range of concentration from 0.5 to 100 ng mL−1 (R2 > 0.995). Recoveries of this method estimated at three spiked concentrations of 100, 250 and 500 ng mL−1 in pig hair samples, were 79–82% for ractopamine and 77–96% for salbutamol. The corresponding inter-day and intra-day precisions expressed as relative standard deviation (RSD %) were 3.8–6.4% and 3.8–8.6%, respectively. The analytical time for one sample was 8 min. The detection limit of this method was 0.6 and 8.3 ng mL−1 for ractopamine and salbutamol, respectively. This developed method can be applied for monitoring the use of the β-agonists salbutamol and ractopamine in swine feed incurred pig hair. Keywords: Salbutamol, Ractopamine, Hair, Tandem mass |
| Databáze: | OpenAIRE |
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