A nuclear casein kinase 2 activity is involved in early events of transcriptional activation induced by salicylic acid in tobacco
| Autor: | Loreto Holuigue, Héctor Ojeda, Xavier Jordana, Perla C. Hidalgo, Carmen Gloria Berrı́os, Virginia Garretón |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Transcriptional Activation
Physiology Nicotiana tabacum GUS reporter system Plant Science Protein Serine-Threonine Kinases Transcription (biology) Tobacco Genetics Protein phosphorylation Amino Acid Sequence Nuclear protein Protein kinase A Casein Kinase II Transcription factor DNA Primers Glucuronidase Cell Nucleus Binding Sites biology Base Sequence fungi biology.organism_classification Molecular biology Peptide Fragments Plant Leaves Biochemistry Casein kinase 2 Salicylic Acid Research Article |
| Zdroj: | PLANT PHYSIOLOGY Artículos CONICYT CONICYT Chile instacron:CONICYT |
| Popis: | Salicylic acid (SA) activates immediate early transcription of genes controlled by a family of DNA promoter elements namedas-1-like elements. These elements are functional in the promoter of glutathione S-transferase genes. We have previously shown that SA increases the binding of tobacco (Nicotiana tabacum cv Xanthi nc) nuclear factors to theas-1 sequence in a process mediated by protein phosphorylation. In this study we give evidence for the participation of a nuclear protein kinase CK2 (casein kinase 2) in the pathway activated by SA in tobacco. The first line of evidence comes from the evaluation of the CK2 activity in nuclear extracts prepared from tobacco plants treated with SA or water as a control. Results from these experiments indicate that SA increases the nuclear CK2 activity. The second line of evidence derives from the evaluation of the in vivo effect of 5,6-dichloro-1-(β-d-ribofuranosyl) benzimidazole (DRB), a cell-permeable CK2 inhibitor, on the responsiveness of the as-1 sequence to SA. Results from these experiments indicate that DRB impairs the activating effect of SA on the transcription of both, the GUS reporter gene controlled by a tetramer of the as-1 element, and the endogenous gnt35 gene encoding a glutathioneS-transferase, in transgenic tobacco plants. DRB also impaired the increasing effect of SA on the binding of nuclear factors to the as-1 element. Furthermore, transcription of theas-1/GUS reporter gene activated by the synthetic auxin 2,4-dichlorophenoxyacetic acid and by methyl jasmonate was also inhibited by DRB. To our knowledge, this is the first report in which activation of a CK2 enzyme by a plant hormone is reported. |
| Databáze: | OpenAIRE |
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