| Popis: |
Misregulation of candidate stem cell marker ASPM, and itsDrosophilahomologue Asp, leads to either tumour formation or microcephaly, but the functional roles contributing to each are not understood. We reverse-engineered flies to express a version of Asp (AspLIE), predicted to have lost its ability to bind the phosphatase PP2A-B’. Although AspLIEflies were viable, they exhibited splayed neural stem cell spindle poles under stress, and development was substantially delayed. A tissue-level analysis of microcephaly and midgut abnormalities in Asp mutants with a compromised spindle assembly checkpoint (SAC) demonstrates tissue-specific vulnerability to mitotic defects. |
