Isolation of a gene that is involved in Campylobacter jejuni 81116 cytotoxin activation
| Autor: | Peter J. Coloe, Benjamin N. Fry, Kaiyan Liu |
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| Rok vydání: | 2007 |
| Předmět: |
Microbiology (medical)
DNA Bacterial Immunology Blotting Western Molecular Sequence Data Restriction Mapping CHO Cells Microbiology Campylobacter jejuni Polymerase Chain Reaction Bacterial genetics Mice Open Reading Frames Cricetulus Neutralization Tests Cricetinae Campylobacter Infections Immunology and Allergy Animals Humans Gene Antiserum Mice Inbred BALB C biology Base Sequence Cytotoxins Wild type General Medicine Periplasmic space bacterial infections and mycoses biology.organism_classification Cosmids Open reading frame Infectious Diseases Cosmid Female HeLa Cells |
| Zdroj: | FEMS immunology and medical microbiology. 49(1) |
| ISSN: | 0928-8244 |
| Popis: | Cytotoxin fractions were isolated from Campylobacter jejuni 81116 and semi-purified by size-exclusion liquid chromatography. The fraction showing the strongest toxicity was injected into mice to produce antiserum. The antiserum was used to screen a C. jejuni 81116 cosmid library. Nine genes were identified in overlapping cosmid inserts that induced reactivity with the antiserum. One of these genes showed high similarity to a periplasmic protein of unknown function and its isogenic mutant showed decreased toxicity compared to the C. jejuni 81116 wild type. This gene contains a Gram-negative bacterial RTX toxin-activating protein C signature, which suggests it may play a role in C. jejuni 81116 cytotoxin activation. |
| Databáze: | OpenAIRE |
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