Clinical experience with preimplantation genetic diagnosis and intracytoplasmic sperm injection
| Autor: | C. Staessen, Hubert Joris, Willy Lissens, M. Vandervorst, Ingeborg Liebaers, Karen Sermon, Paul Devroey, A. Van Steirteghem, Maryse Bonduelle, E. Van Assche, Peter B. Nagy |
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| Přispěvatelé: | Tarlatzis, Basil, Department of Embryology and Genetics, Vrije Universiteit Brussel |
| Rok vydání: | 1998 |
| Předmět: |
Adult
Male medicine.medical_specialty Genotype Microinjections Pregnancy Rate medicine.medical_treatment Prenatal diagnosis Fertilization in Vitro Biology Preimplantation genetic diagnosis Polymerase Chain Reaction Intracytoplasmic sperm injection Human fertilization FISH Pregnancy medicine Humans In Situ Hybridization Fluorescence Preimplantation Diagnosis PGD Obstetrics Rehabilitation Genetic Diseases Inborn Pregnancy Outcome Obstetrics and Gynecology Embryo Embryo Transfer medicine.disease Spermatozoa Embryo transfer Pregnancy rate PCR Reproductive Medicine IVF Female |
| Zdroj: | Human Reproduction. 13:186-195 |
| ISSN: | 1460-2350 0268-1161 |
| Popis: | Preimplantation genetic diagnosis (PGD) is a novel procedure whose use may be considered to obtain a very early prenatal diagnosis for couples at risk for transmitting genetic diseases. Using the polymerase chain reaction (PCR) or fluorescence in-situ hybridization (FISH) the genotype or the sex of biopsied cleavage-stage embryos obtained after in-vitro fertilization can be determined and selected embryos can then be transferred. In-vitro fertilization with intracytoplasmic sperm injection is the method of choice to obtain embryos to be analysed through PCR to reduce contamination by residual sperm DNA. In our series of 61 PGD cycles for 29 couples at risk over a period of 4 years the ongoing pregnancy rate per cycle was 15%, per transfer 19% and per patient 31%. One of the six morphologically normal children born, who is still alive and doing well, weighed 850 g after birth at 25 weeks following a complicated triplet pregnancy. More experience is needed to correctly evaluate the efficiency and safety of this novel technique as well as to determine its place in the prevention of genetic disease. |
| Databáze: | OpenAIRE |
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