Isolation and transcriptome analysis of adult zebrafish cells enriched for skeletal muscle progenitors
| Autor: | Leonard I. Zon, Genri Kawahara, Timothy J. Pusack, Matthew S. Alexander, Jeffrey R. Guyon, Melanie H. Howell, Jennifer Myers, Federica Montanaro, Alvin T. Kho, Louis M. Kunkel |
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| Rok vydání: | 2011 |
| Předmět: |
Aging
Physiology Cellular differentiation Muscle Development Animals Genetically Modified Myoblasts myogenic progenitors Transcriptome 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine muscle mutants Myotome Physiology (medical) medicine Animals Myocyte Muscle Skeletal Zebrafish Cells Cultured 030304 developmental biology 0303 health sciences biology Myogenesis Gene Expression Profiling Stem Cells Main Articles Skeletal muscle Cell Differentiation differentiation zebrafish biology.organism_classification Molecular biology 3. Good health muscle stem cells medicine.anatomical_structure myogenesis Neurology (clinical) Stem cell transcriptome 030217 neurology & neurosurgery |
| Zdroj: | Muscle & Nerve |
| ISSN: | 1097-4598 0148-639X |
| DOI: | 10.1002/mus.21972 |
| Popis: | Introduction: Over the past 10 years, the use of zebrafish for scientific research in the area of muscle development has increased dramatically. Although several protocols exist for the isolation of adult myoblast progenitors from larger fish, no standardized protocol exists for the isolation of myogenic progenitors from adult zebrafish muscle. Methods: Using a variant of a mammalian myoblast isolation protocol, zebrafish muscle progenitors have been isolated from the total dorsal myotome. These zebrafish myoblast progenitors can be cultured for several passages and then differentiated into multinucleated, mature myotubes. Results: Transcriptome analysis of these cells during myogenic differentiation revealed a strong downregulation of pluripotency genes, while, conversely, showing an upregulation of myogenic signaling and structural genes. Conclusions: Together these studies provide a simple, yet detailed method for the isolation and culture of myogenic progenitors from adult zebrafish, while further promoting their therapeutic potential for the study of muscle disease and drug screening. Muscle Nerve 43: 741–750, 2011 |
| Databáze: | OpenAIRE |
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