Elemental Bioimaging of Nanosilver-Coated Prostheses Using X-ray Fluorescence Spectroscopy and Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry
| Autor: | Steffen Höll, Uwe Karst, Michael R. Sperling, Georg Gosheger, Olga Reifschneider, Gregor Hauschild, Franziska Blaske, Christoph A. Wehe |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Detection limit
chemistry.chemical_classification Silver medicine.diagnostic_test Chemistry Spectrophotometry Atomic Analytical chemistry Metal Nanoparticles Spectrometry X-Ray Emission Vanadium chemistry.chemical_element Polymer Bone tissue Mass spectrometry Mass Spectrometry Analytical Chemistry Dogs medicine.anatomical_structure Spectrophotometry medicine Animals Hip Prosthesis Laser Therapy Cancellous bone Titanium |
| Zdroj: | Analytical Chemistry. 86:615-620 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/ac4028577 |
| Popis: | The distribution of different chemical elements from a nanosilver-coated bone implant was visualized, combining the benefits of two complementary methods for elemental bioimaging, the nondestructive micro X-ray fluorescence (μ-XRF), and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Challenges caused by the physically inhomogeneous materials including bone and soft tissues were addressed by polymer embedding. With the use of μ-XRF, fast sample mapping was achieved obtaining titanium and vanadium signals from the metal implant as well as phosphorus and calcium signals representing hard bone tissue and sulfur distribution representing soft tissues. Only by the use of LA-ICP-MS, the required high sensitivity and low detection limits for the determination of silver were obtained. Metal distribution within the part of cancellous bone was revealed for silver as well as for the implant constituents titanium, vanadium, and aluminum. Furthermore, the detection of coinciding high local zirconium and aluminum signals at the implant surface indicates remaining blasting abrasive from preoperative surface treatment of the nanosilver-coated device. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|