Carbodiimide Inactivation of MMPs and Effect on Dentin Bonding
| Autor: | Vittorio Checchi, Valeria Angeloni, Annalisa Mazzoni, David H. Pashley, Lorenzo Breschi, Fm Apolonio, Rosa Curci, Spartaco Santi, Vicente de Paulo Aragão Saboia, R. Di Lenarda, Franklin Chi Meng Tay |
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| Přispěvatelé: | Mazzoni, Annalisa, F. M., Apolonio, V. P. A., Saboia, S., Santi, Angeloni, Valeria, Checchi, Vittorio, R., Curci, DI LENARDA, Roberto, F. R., Tay, D. H., Pashley, Breschi, Lorenzo, Mazzoni A, Apolonio FM, Saboia VP, Santi S, Angeloni V, Checchi V, Curci R, Di Lenarda R, Tay FR, Pashley DH, Breschi L |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
In situ
Gelatinases collagen cross-linker Dentistry adhesive system Matrix metalloproteinase Matrix Metalloproteinase Inhibitors adhesive systems biochemical assays dentin bonding agents endogenous proteinases human dentin chemistry.chemical_compound In situ zymography stomatognathic system Acid Etching Dental Ethyldimethylaminopropyl Carbodiimide Dentin medicine dentin bonding systems Humans Zymography Phosphoric Acids General Dentistry biochemical assay Carbodiimide Fluorescent Dyes Chromatography business.industry Dental Bonding Research Reports Resin Cements endogenous proteinase medicine.anatomical_structure Cross-Linking Reagents chemistry Matrix Metalloproteinase 9 Dentin-Bonding Agents Matrix Metalloproteinase 2 Fluorescein Adhesive business dentin bonding agent |
| Popis: | The use of protein cross-linking agents during bonding procedures has been recently proposed to improve bond durability. This study aimed to use zymography and in situ zymography techniques to evaluate the ability of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) cross-linker to inhibit matrix metalloproteinase (MMP) activity. The hypotheses tested were that: (1) bonding procedures increase dentin gelatinolytic activity and (2) EDC pre-treatment prevents this enzymatic activity. The zymographic assay was performed on protein extracts obtained from dentin powder treated with Optibond FL or Scotchbond 1XT with or without 0.3M EDC pre-treatment. For in situ zymography, adhesive/dentin interfaces were created with the same adhesives applied to acid-etched dentin slabs pre-treated or not with EDC conditioner. Zymograms revealed increased expression of dentin endogenous MMP-2 and -9 after adhesive application, while the use of EDC as a primer inactivated dentin gelatinases. Results of in situ zymograpy showed that hybrid layers of tested adhesives exhibited intense collagenolytic activity, while almost no fluorescence signal was detected when specimens were pre-treated with EDC. The correlative analysis used in this study demonstrated that EDC could contribute to inactivate endogenous dentin MMPs within the hybrid layer created by etch-and-rinse adhesives. |
| Databáze: | OpenAIRE |
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