High degree of concordance between flow cytometry and geno2pheno methods for HIV-1 tropism determination in proviral DNA
| Autor: | Gilcivaldo de Jesus Ferreira, Luana Portes Ozório Coelho, Alex José Leite Torres, Ana Luiza Dias Angelo, Eduardo Martins Netto, Célia Regina Mayoral Pedroso Jorge, Marcos Herculano Nunes Abrahão, Luís Fernando de Macedo Brígido, João Leandro de Paula Ferreira, Carlos Brites |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Microbiology (medical)
CD4-Positive T-Lymphocytes Genotype medicine.drug_class viruses lcsh:QR1-502 HIV Infections Biology CD8-Positive T-Lymphocytes Monoclonal antibody Tropism Peripheral blood mononuclear cell Sensitivity and Specificity lcsh:Microbiology Flow cytometry lcsh:Infectious and parasitic diseases Predictive Value of Tests medicine Geno2pheno Humans lcsh:RC109-216 Medicine(all) medicine.diagnostic_test virus diseases Viral Load Flow Cytometry Virology Molecular biology Viral Tropism Infectious Diseases Phenotype DNA Viral Tissue tropism HIV-1 Leukocytes Mononuclear Viral load Cytometry Algorithms |
| Zdroj: | Brazilian Journal of Infectious Diseases, Vol 19, Iss 2, Pp 163-169 (2015) |
| ISSN: | 1413-8670 |
| Popis: | Use of CCR5 antagonists requires previous viral tropism determination. The available methods have high cost, are time-consuming, or require highly trained personnel, and sophisticated equipment. We compared a flow cytometry-based tropism assay with geno2pheno method to determine HIV-1 tropism in AIDS patients, in Bahia, Brazil. We tested peripheral blood mononuclear cells of 102 AIDS patients under antiretroviral therapy by using a cytometry-based tropism assay and geno2pheno assay. Cellular membrane receptors were identified by using CXCR4, CCR5 and CD4 monoclonal antibodies, while detection of cytoplasmic mRNAs for gag and pol HIV regions was achieved by using a labeled probe. Genotypic identification of X4 and R5 tropic viruses was attempted by geno2pheno algorithm. There was a high degree of concordance between cytometry-based tropism assay and geno2pheno algorithm in determination of HIV-1 tropism. Cytometry-based tropism assay demonstrated higher sensitivity and specificity in comparison to geno2pheno, which was used as a gold-standard. One sample could not be amplified by geno2pheno method, but was classified as duotropic by cytometry-based tropism assay. We did not find any association between CD4+ count or plasma HIV-1 RNA viral load and tropism results. The overall performances of cytometry-based tropism assay and geno2pheno assay were almost identical in determination of HIV-1 tropism. Keywords: HIV-1, Tropism, Flow cytometry, Geno2pheno |
| Databáze: | OpenAIRE |
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