Peculiarities of Cyto- and Chemoarchitectonics of Human Entorhinal Cortex during the Fetal Period
| Autor: | E A Tsvetkov, L. A. Tkachenko, Ruslan A. Nasyrov, I A Moiseenko, P. A. Zykin, E. I. Krasnoshchekova |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Calbindins Gestational Age Calbindin General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Asphyxia 0302 clinical medicine Fetus Neurofilament Proteins Calcium-binding protein Cortex (anatomy) medicine Image Processing Computer-Assisted Entorhinal Cortex Humans Reelin Neurons biology Chemistry Colocalization Gene Expression Regulation Developmental General Medicine Microtomy Entorhinal cortex Immunohistochemistry Cell biology Reelin Protein 030104 developmental biology medicine.anatomical_structure Parvalbumins nervous system Calbindin 2 biology.protein Calretinin Microtubule-Associated Proteins 030217 neurology & neurosurgery Parvalbumin |
| Zdroj: | Bulletin of experimental biology and medicine. 164(4) |
| ISSN: | 1573-8221 |
| Popis: | We studied peculiarities of the structure of human entorhinal cortex at weeks 20-26 of gestation (10 hemispheres). The samples were Nissl-stained and immunohistochemically treated with antibodies to parvalbumin, calretinin, calbindin, and cytoskeleton proteins (MAP2 and N200). 3D-reconstruction of the entorhinal cortex from serial sections was performed, caudomedial and rostrolateral areas were isolated. Parvalbumin+ cells in layer I, discrete distribution of layer II cells with colocalization of MAP2 and calretinin at the border with layer I, and two sublayers Va and Vb with MAP2+ neurons were typical for the caudomedial area. Rostrolateral area was characterized by the homogenous layer II with big amount of cells, high density of MAP2+ neurons only in layer III, and the unique layer V. Reelin+ Cajal-Retzius cells and N200+ fiber plexus in layer I were observed in the caudomedial and rostrolateral areas of the entorhial cortex. Layer IV was represented by a cell-free desiccant. |
| Databáze: | OpenAIRE |
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