Analysis of SRF, SAP-1 and ELK-1 transcripts and proteins in human cell lines
| Autor: | Marc Lipinski, Laura Magnaghi-Jaulin, Hiroshi Masutani, Annick Harel-Bellan |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Serum Response Factor
DNA Complementary Blotting Western Molecular Sequence Data Restriction Mapping Biophysics Biology Biochemistry Cell Line Structural Biology Serum responsive element Proto-Oncogene Proteins Serum response factor Tumor Cells Cultured Genetics Transcriptional regulation Humans RNA Messenger ets-Domain Protein Elk-4 Northern blot Molecular Biology Gene Transcription factor ets-Domain Protein Elk-1 ELK-1 Base Sequence SAP-1 Nuclear Proteins RNA Cell Biology Serum responsive factor Blotting Northern Molecular biology DNA-Binding Proteins Blot Cell culture Electrophoresis Polyacrylamide Gel HeLa Cells Transcription Factors |
| Zdroj: | FEBS Letters. (3):247-251 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(96)00745-4 |
| Popis: | We have analysed the expression of the genes encoding transcription factors involved in c - fos transcriptional regulation, i.e. the serum response factor (SRF) and the ETS-related proteins ELK-1 and SAP-1, in a variety of human cell lines. RNA was determined by Northern blot analysis, and proteins were detected on Western blots: the two analyses gave essentially identical results. SRF was expressed at similar levels in all cell lines tested. In contrast, SAP-1 and ELK-1 expression varied from one cell line to another. Interestingly, in any given cell line, high levels of one protein were accompanied by low levels of the other. Similar results were obtained by electromobility shift assays (EMSA), using antibodies directed against the proteins. Thus, our data raise the possibility of a coordinated regulation of the expression of these two Ets genes, at both RNA and protein levels. |
| Databáze: | OpenAIRE |
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