The basic framework of VE-cadherin junctions revealed by cryo-EM

Autor:	Alain Brisson, Jean-Christophe Taveau, Rana Al Kurdi, Josephine Lai Kee Him, Olivier Lambert, Danielle Gulino-Debrac
Rok vydání:	2004
Předmět:	Cadherin Cryo-electron microscopy Cryoelectron Microscopy macromolecular substances Adherens Junctions Biology Cadherins Cell biology Adherens junction Structural Biology Antigens CD Liposomes Extracellular Humans Endothelium Vascular VE-cadherin Molecular Biology Cell Adhesion Molecules
Zdroj:	Journal of molecular biology. 346(5)
ISSN:	0022-2836
Popis:	Artificial adherens junctions were reconstituted in vitro by assembly of cadherin fragments at the surfaces of liposomes. The architecture of the adherens junctions was revealed by cryo-electron microscopy (cryo-EM). The formation of these artificial adherens junctions was shown to result from the two-dimensional (2D) self-assembly of cadherin fragments at membrane surfaces. The molecular architecture of the junctions was resolved by combining information from several cryo-EM views. This study concludes to the 2D ordered nature of the cadherin assembly and shows that the minimal information required to build up an adherens junction is contained within the extracellular moiety of cadherin molecules.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::215ba82a083bde059f6033a098e57dcb https://pubmed.ncbi.nlm.nih.gov/15713473 Zobrazit plný text záznamu Full Text from ScienceDirect