Dark-induced mRNA instability involves RNase E/G-type endoribonuclease cleavage at the AU-box and SD sequences in cyanobacteria
| Autor: | Miyuki Ono, Munehiko Asayama, Makoto Shirai, Yuriko Hamakubo, Yoshinao Horie, Hideki Kato, Tomoki Amano, Yoko Ito, Naoko Moriwaki, Masaaki Wachi |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
RNase P
RNA Stability Endoribonuclease Biology RNase PH Bacterial Proteins Endoribonucleases Gene expression Escherichia coli Genetics RNA Messenger Ribonuclease Molecular Biology Binding Sites Base Sequence Models Genetic Genetic Complementation Test Synechocystis Photosystem II Protein Complex General Medicine Darkness biology.organism_classification Molecular biology Recombinant Proteins RNA Bacterial RNase MRP Genes Bacterial Mutagenesis Site-Directed biology.protein Nucleic Acid Conformation Degradosome |
| Zdroj: | Molecular Genetics and Genomics. 278:331-346 |
| ISSN: | 1617-4623 1617-4615 |
| DOI: | 10.1007/s00438-007-0254-9 |
| Popis: | Light-responsive gene expression is crucial to photosynthesizing organisms. Here, we studied functions of cis-elements (AU-box and SD sequences) and a trans-acting factor (ribonuclease, RNase) in light-responsive expression in cyanobacteria. The results indicated that AU-rich nucleotides with an AU-box, UAAAUAAA, just upstream from an SD confer instability on the mRNA under darkness. An RNase E/G homologue, Slr1129, of the cyanobacterium Synechocystis sp. strain PCC 6803 was purified and confirmed capable of endoribonucleolytic cleavage at the AU- (or AG)-rich sequences in vitro. The cleavage depends on the primary target sequence and secondary structure of the mRNA. Complementation tests using Escherichia coli rne/rng mutants showed that Slr1129 fulfilled the functions of both the RNase E and RNase G. An analysis of systematic mutations in the AU-box and SD sequences showed that the cis-elements also affect significantly mRNA stability in light-responsive genes. These results strongly suggested that dark-induced mRNA instability involves RNase E/G-type cleavage at the AU-box and SD sequences in cyanobacteria. The mechanical impact and a possible common mechanism with RNases for light-responsive gene expression are discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink