Yeast nuclear envelope subdomains with distinct abilities to resist membrane expansion
| Autor: | Joseph L. Campbell, Josef Loidl, Orna Cohen-Fix, Thomas Hays, Keren L. Witkin, Alexander Lorenz |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Nuclear Envelope Saccharomyces cerevisiae Biology Endoplasmic Reticulum DNA Ribosomal medicine Inner membrane Nuclear membrane Nuclear protein Molecular Biology Phospholipids Cell Nucleus Endoplasmic reticulum Membrane Proteins Nuclear Proteins RNA-Binding Proteins Articles Cell Biology Cell biology Cell nucleus medicine.anatomical_structure Membrane protein Mutation Nuclear lamina Chromosomes Fungal Cell Nucleolus Gene Deletion Lamin |
| Popis: | Little is known about what dictates the round shape of the yeast Saccharomyces cerevisiae nucleus. In spo7Delta mutants, the nucleus is misshapen, exhibiting a single protrusion. The Spo7 protein is part of a phosphatase complex that represses phospholipid biosynthesis. Here, we report that the nuclear protrusion of spo7Delta mutants colocalizes with the nucleolus, whereas the nuclear compartment containing the bulk of the DNA is unaffected. Using strains in which the nucleolus is not intimately associated with the nuclear envelope, we show that the single nuclear protrusion of spo7Delta mutants is not a result of nucleolar expansion, but rather a property of the nuclear membrane. We found that in spo7Delta mutants the peripheral endoplasmic reticulum (ER) membrane was also expanded. Because the nuclear membrane and the ER are contiguous, this finding indicates that in spo7Delta mutants all ER membranes, with the exception of the membrane surrounding the bulk of the DNA, undergo expansion. Our results suggest that the nuclear envelope has distinct domains that differ in their ability to resist membrane expansion in response to increased phospholipid biosynthesis. We further propose that in budding yeast there is a mechanism, or structure, that restricts nuclear membrane expansion around the bulk of the DNA. |
| Databáze: | OpenAIRE |
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