Aqueous Extract of Curcuma aromatica Induces Apoptosis and G2/M Arrest in Human Colon Carcinoma LS-174-T Cells Independent of p53
| Autor: | Hong-Mei An, Ke-Ping Shen, Qin Du, Yang Wu, Bing Hu |
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| Rok vydání: | 2011 |
| Předmět: |
G2 Phase
Cancer Research Cell Curcuma aromatica Apoptosis Pharmacology Curcuma Cell Line Tumor CDC2 Protein Kinase Carcinoma medicine Humans Radiology Nuclear Medicine and imaging Cyclin B1 Tumor Stem Cell Assay Caspase Traditional medicine biology Plant Extracts Cell growth General Medicine Cell cycle biology.organism_classification medicine.disease Antineoplastic Agents Phytogenic Caspase Inhibitors Enzyme Activation medicine.anatomical_structure Oncology Cell culture Caspases Colonic Neoplasms Neoplastic Stem Cells biology.protein Tumor Suppressor Protein p53 Cell Division |
| Zdroj: | Cancer Biotherapy and Radiopharmaceuticals. 26:97-104 |
| ISSN: | 1557-8852 1084-9785 |
| DOI: | 10.1089/cbr.2010.0853 |
| Popis: | Curcuma aromatica is a common Chinese herb for treating diseases with blood stasis and has been regarded as an anticancer herb in modern clinical practice. However, the anticancer effects and related molecular mechanisms of Curcuma aromatica remain unclear. In the present study, human colon carcinoma LS-174-T cell line with wild-type p53 was used as a model cell to evaluate the anticancer effects of aqueous extract of Curcuma aromatica (AECA). AECA inhibits LS-174-T cell proliferation in a dose- and time-dependent manner and colony formation in a dose-dependent manner. AECA treatment induces apoptosis accompanied by caspase-8, -9, and -3 activation in LS-174-T cells. Moreover, blocking the activities of these caspases with a specific inhibitor significantly protected LS-174-T cells from AECA-induced apoptosis. AECA treatment also induces G2/M phase arrest in LS-174-T cells. Expression of p53 was unchanged after AECA treatment; specific silence of p53 did not influence AECA-induced apoptosis and G2/M phase arrest. Further, the expression of cyclin B1 and CDK1 was reduced by AECA. This study suggests that AECA might be effective as an antiproliferative herb for colon carcinoma, the antitumor activity of AECA may involve both extrinsic and intrinsic apoptosis, and AECA induces G2/M phase arrest via downregulation of cyclin B1 and CDK1 and without the participation of p53. |
| Databáze: | OpenAIRE |
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