Initial cord blood unit volume affects mononuclear cell and CD34+ cell-processing efficiency in a non-linear fashion
| Autor: | André Tichelli, Eddy Roosnek, Yves Chalandon, Sandrine Meyer-Monard, Daniel Surbek, Olivier Irion, Damiano Castelli, Grazia Nicoloso de Faveri, Alois Gratwohl, Carolyn Troeger, Jakob Passweg, Vincent Kindler, Caroline Arber |
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| Rok vydání: | 2011 |
| Předmět: |
Cancer Research
T-Lymphocytes Immunology Cell Culture Techniques Cell Culture Techniques/methods Antigens CD34 Blood Sedimentation Hydroxyethyl starch Umbilical cord Peripheral blood mononuclear cell Hydroxyethyl Starch Derivatives fluids and secretions Fetal Blood/cytology medicine Immunology and Allergy Humans Genetics (clinical) Hetastarch/chemistry ddc:616 Transplantation Leukocytes Mononuclear/cytology ddc:618 Hematopoietic Stem Cells/cytology business.industry Processing efficiency Hematopoietic stem cell Cell Biology Fetal Blood Hematopoietic Stem Cells Haematopoiesis medicine.anatomical_structure Oncology Cord blood embryonic structures Cancer research Leukocytes Mononuclear Antigens CD34/immunology Stem cell T-Lymphocytes/cytology/immunology business medicine.drug |
| Zdroj: | Cytotherapy, Vol. 14, No 2 (2012) pp. 215-22 |
| ISSN: | 1477-2566 1465-3249 |
| Popis: | Umbilical cord blood (UCB) is a source of hematopoietic stem cells that initially was used exclusively for the hematopoietic reconstitution of pediatric patients. It is now suggested for use for adults as well, a fact that increases the pressure to obtain units with high cellularity. Therefore, the optimization of UCB processing is a priority.The present study focused on parameters influencing total nucleated cell (TNC), mononucleated cell (MNC) and CD34+ cell (CD34C) recovery after routine volume reduction of 1553 UCB units using hydroxyethyl starch-induced sedimentation with an automated device, under routine laboratory conditions.We show that the unit volume rather than the TNC count significantly affects TNC, MNC and CD34C processing efficiency (PEf), and this in a non-linear fashion: when units were sampled according to the collection volume, including pre-loaded anticoagulant (gross volume), PEf increased up to a unit volume of 110-150 mL and decreased thereafter. Thus units with initial gross volumes90 mL and170 mL similarly exhibited a poor PEf.These data identify unit gross volume as a major parameter influencing PEf and suggest that fractionation of large units should be contemplated only when the resulting volume of split units is90 mL. |
| Databáze: | OpenAIRE |
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