Dietary Selenium Levels Affect Selenoprotein Expression and Support the Interferon-γ and IL-6 Immune Response Pathways in Mice
| Autor: | Michael T. Howard, Dolph L. Hatfield, Christine B. Anderson, Harold E. Seifried, Bradley A. Carlson, Petra A. Tsuji |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Microarray
Gene Expression selenoprotein Mice 0302 clinical medicine Selenium deficiency Gene expression Interferon gamma Selenoproteins chemistry.chemical_classification 0303 health sciences Nutrition and Dietetics IL-6 interferon-γ ribosome profiling selenium selenocysteine food and beverages 3. Good health Up-Regulation Biochemistry 030220 oncology & carcinogenesis lcsh:Nutrition. Foods and food supply medicine.drug inorganic chemicals medicine.medical_specialty chemistry.chemical_element lcsh:TX341-641 Biology Article 03 medical and health sciences Interferon-gamma Selenium Immune system Sodium Selenite Internal medicine medicine Animals RNA Messenger 030304 developmental biology Inflammation Interleukin-6 Sequence Analysis RNA Gene Expression Profiling Computational Biology medicine.disease Gene expression profiling Endocrinology chemistry Dietary Supplements Selenoprotein Food Science |
| Zdroj: | Nutrients Nutrients; Volume 7; Issue 8; Pages: 6529-6549 Nutrients, Vol 7, Iss 8, Pp 6529-6549 (2015) Volume 7 Issue 8 Pages 6529-6549 |
| ISSN: | 2072-6643 |
| Popis: | Selenium is an essential element that is required to support a number of cellular functions and biochemical pathways. The objective of this study was to examine the effects of reduced dietary selenium levels on gene expression to assess changes in expression of non-selenoprotein genes that may contribute to the physiological consequences of selenium deficiency. Mice were fed diets that were either deficient in selenium or supplemented with selenium in the form of sodium selenite for six weeks. Differences in liver mRNA expression and translation were measured using a combination of ribosome profiling, RNA-Seq, microarrays, and qPCR. Expression levels and translation of mRNAs encoding stress-related selenoproteins were shown to be up-regulated by increased selenium status, as were genes involved in inflammation and response to interferon-γ. Changes in serum cytokine levels were measured which confirmed that interferon-γ, as well as IL-6, were increased in selenium adequate mice. Finally, microarray and qPCR analysis of lung tissue demonstrated that the selenium effects on immune function are not limited to liver. These data are consistent with previous reports indicating that adequate selenium levels can support beneficial immune responses, and further identify the IL-6 and interferon-γ pathways as being responsive to dietary selenium intake. |
| Databáze: | OpenAIRE |
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