Galectin-9 Induced by Dietary Synbiotics is Involved in Suppression of Allergic Symptoms in Mice and Humans
| Autor: | S. de Kivit, H. J. G. van de Kant, Eirikur Saeland, Aletta D. Kraneveld, Linette E M Willemsen, B.C.A.M. van Esch, Léon M.J. Knippels, L. B. van der Aa, Jan Knol, Y. (Yvette) van Kooyk, Johan Garssen, Bastiaan Schouten, Aline B. Sprikkelman |
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| Přispěvatelé: | Amsterdam institute for Infection and Immunity, Paediatric Pulmonology, General Paediatrics, CCA - Immuno-pathogenesis, NCA - Multiple Sclerosis and Other Neuroinflammatory Diseases, Molecular cell biology and Immunology, Pediatric surgery |
| Předmět: |
Allergy
Bifidobacterium breve Cell Degranulation ved/biology Immunology ved/biology.organism_classification_rank.species Degranulation Inflammation Biology medicine.disease Peripheral blood mononuclear cell medicine.anatomical_structure medicine Immunology and Allergy Mesenteric lymph nodes medicine.symptom Galectin |
| Zdroj: | ResearcherID Allergy, 67(3), 343-352. Wiley-Blackwell de Kivit, S, Saeland, E, Kraneveld, A D, van de Kant, H J G, Schouten, B, van Esch, B C A M, Knol, J, Sprikkelman, A B, van der Aa, L B, Knippels, L M J, Garssen, J, van Kooyk, Y & Willemsen, L E M 2012, ' Galectin-9 induced by dietary synbiotics is involved in suppression of allergic symptoms in mice and humans ', Allergy, vol. 67, no. 3, pp. 343-352 . https://doi.org/10.1111/j.1398-9995.2011.02771.x |
| ISSN: | 0105-4538 |
| DOI: | 10.1111/j.1398-9995.2011.02771.x |
| Popis: | Background: Prebiotic galacto- and fructo-oligosaccharides (scGOS/lcFOS) resembling non-digestible oligosaccharides in human milk reduce the development of atopic disorders. However, the underlying mechanisms are still unclear. Galectins are soluble-type lectins recognizing β-galactoside containing glycans. Galectin-9 has been shown to regulate mast cell degranulation and T-cell differentiation. In this study, the involvement of galectin-9 as a mechanism by which scGOS/lcFOS in combination with Bifidobacterium breve M-16V protects against acute allergic symptoms was investigated. Methods: Mice were sensitized orally to whey, while being fed with a diet containing scGOS/lcFOS and Bifidobacterium breve M-16V (GF/Bb) or a control diet. Galectin-9 expression was determined by immunohistochemistry in the intestine and measured in the serum by ELISA. T-cell differentiation was investigated in the mesenteric lymph nodes (MLN) as well as in galectin-9-exposed peripheral blood mononuclear cells (PBMC) cultures. Sera of the mice were evaluated for the capacity to suppress mast cell degranulation using a RBL-2H3 degranulation assay. In addition, in a double-blind, placebo-controlled multicenter trial, galectin-9 levels were measured in the sera of 90 infants with atopic dermatitis who received hydrolyzed formulae with or without GF/Bb. Results: Galectin-9 expression by intestinal epithelial cells and serum galectin-9 levels were increased in mice and humans following dietary intervention with GF/Bb and correlated with reduced acute allergic skin reaction and mast cell degranulation. In addition, GF/Bb enhanced Th1- and Treg-cell differentiation in MLN and in PBMC cultures exposed to galectin-9. Conclusions: Dietary supplementation with GF/Bb enhances serum galectin-9 levels, which associates with the prevention of allergic symptoms. |
| Databáze: | OpenAIRE |
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