Ontogeny of sulfonylurea-binding regulatory subunits of KATP channels in the pregnant rat myometrium
Autor: | George Falkay, Norbert Lovasz, Róbert Gáspár, Eszter Ducza |
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Rok vydání: | 2011 |
Předmět: |
Embryology
medicine.medical_specialty Tocolytic agent Receptors Drug In Vitro Techniques Pregnancy Proteins Oxytocin Sulfonylurea Receptors Rats Sprague-Dawley Glibenclamide Uterine Contraction chemistry.chemical_compound Endocrinology KATP Channels Pregnancy Internal medicine Potassium Channel Blockers medicine Diazoxide Animals Protein Isoforms Channel blocker RNA Messenger Potassium Channels Inwardly Rectifying Chemistry Pinacidil Myometrium Obstetrics and Gynecology Cell Biology Potassium channel Rats Protein Subunits Sulfonylurea Compounds Tocolytic Agents Gene Expression Regulation Reproductive Medicine Sulfonylurea receptor ATP-Binding Cassette Transporters Female medicine.drug |
Zdroj: | REPRODUCTION. 142:175-181 |
ISSN: | 1741-7899 1470-1626 |
DOI: | 10.1530/rep-10-0492 |
Popis: | ATP-sensitive potassium channels (KATPchannels) are composed of sulfonylurea receptors (SURs) and potassium inward rectifiers (Kir6.x) that assemble to form a large octameric channel. This study was designed to examine the expression and role of sulfonylurea-binding regulatory subunits 1 (SUR1 (ABCC8)) and 2 (SUR2 (ABCC9)) of the KATPchannels in the pregnant rat myometrium with particular regard to the contractility. RT-PCR and western blot analyses were performed to detect the presence of SUR1 and SUR2. The SUR1 levels were markedly increased in the early stages of pregnancy. The highest level was detected on day 6 of pregnancy, whereas in the late stages, the levels of SUR1 were significantly decreased. The SUR2 level remained unchanged throughout pregnancy. The SUR non-selective diazoxide and the SUR2-selective pinacidil inhibited oxytocin-induced contractions. Glibenclamide, a KATPchannel blocker, antagonized both pinacidil- and diazoxide-induced relaxations. It was established that SURs are responsible for pharmacological reactivity of KATPchannel openers. We conclude that both SURs are involved in the KATPchannel in the pregnant rat myometrium. It may further be concluded that ‘pinacidil-like’ KATPchannel openers may be of therapeutic relevance as tocolytic agents in the future. |
Databáze: | OpenAIRE |
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