Asymmetric chromatin retention and nuclear envelopes separate chromosomes in fused cells in vivo

Autor:	Roberto Carlos Segura, Clemens Cabernard, Nicole MynYi Lee, Jennifer Taylor, Chantal Roubinet, Bharath Sunchu
Přispěvatelé:	Lee, Nicole MynYi [0000-0001-6953-2177], Taylor, Jennifer A [0000-0002-8080-9517], Segura, Roberto Carlos [0000-0001-5579-7430], Roubinet, Chantal [0000-0002-9335-4523], Cabernard, Clemens [0000-0003-3706-6401], Apollo - University of Cambridge Repository
Rok vydání:	2022
Předmět:	64 82 Nuclear Envelope 631/80/103 article Medicine (miscellaneous) 13 14 General Biochemistry Genetics and Molecular Biology Chromatin Chromosomes 13/1 64/24 13/100 14/63 Animals Drosophila Proteins Drosophila 631/80 14/19 General Agricultural and Biological Sciences 14/35
Popis:	Hybrid cells derived through fertilization or somatic cell fusion recognize and separate chromosomes of different origins. The underlying mechanisms are unknown but could prevent aneuploidy and tumor formation. Here, we acutely induce fusion between Drosophila neural stem cells (neuroblasts; NBs) and differentiating ganglion mother cells (GMCs) in vivo to define how epigenetically distinct chromatin is recognized and segregated. We find that NB-GMC hybrid cells align both endogenous (neuroblast-origin) and ectopic (GMC-origin) chromosomes at the metaphase plate through centrosome derived dual-spindles. Physical separation of endogenous and ectopic chromatin is achieved through asymmetric, microtubule-dependent chromatin retention in interphase and physical boundaries imposed by nuclear envelopes. The chromatin separation mechanisms described here could apply to the first zygotic division in insects, arthropods, and vertebrates or potentially inform biased chromatid segregation in stem cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::214d13eda29285ded549f052fe70c1ec https://www.repository.cam.ac.uk/handle/1810/342367 Zobrazit plný text záznamu